UVITEC Cambridge FIREREADER MAX User & Service Manual page 47

The factory settings include 3 pre-defined Application protocols:
- SafeLight
- UV fluorescence
- Conversion screen
You can also create or select your own Application protocol. Please refer to the Application protocol
chapter of this manual to have more details on the way you can create your own Application.
2- Click on Start Exposure.
The system will calculate automatically an ideal exposure time. After validation, the system will
expose automatically your sample using the calculated auto exposure time.
Note: The auto-exposure is controlled by a set of parameters described in the Application protocol
chapters of this manual.
Note: With long integration time, a delay could be necessary before an image is displayed on the
monitor. The acquisition will stop automatically at the end of the exposure time.
Note: When the specified exposure time is reached, the last captured image is displayed. The camera
continues to integrate the image on the CCD sensor, updating the display whenever the specified
Exposure time is reached. The Stop exposure button stops the exposure process. The last full
exposure is displayed.
Preview
You can also preview your sample using the preview function (live mode):
Live mode allows direct visualization of the image. This mode enables you to adjust the aperture and
the focus, and to position your sample.
Note: A live image means the image displayed is refreshed every 1/20th of a second. This short
exposure time (or frame) is adequate for a variety of white light samples including protein gels and
autoradiography. A live image, however, is not sufficient for most samples, which are visualized and
photographed over a relatively dim UV light source. A feature called integration compensates the low
light situation by allowing the CCD camera to obtain a timed exposure.
Note: After 2 minutes, the software will automatically stop the live preview.
The stop function captures the last image for further saving, analysis or enhancement. To proceed,
click on the stop preview button.
Set-up
The Set-up button gives you access to the main exposure parameters. These parameters could be
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