Inverse
Click on the "Inverse the image" to inverse the grey level of the image. This makes a negative image.
Positive image
(white spot, black background)
Saturation
A saturated image is inappropriate for image quantification with image analysis software. The saturation
option allows you to visualize in red, pixels that have the maximum grey level in order to avoid flattened
peaks.
The maximum grey level depends of the bit-depth. For instance:
-
A 8-bit image has a maximum grey level of 255.
-
A 12-bit image has a maximum grey level of 4095.
-
A 14-bit image has a maximum grey level of 16383.
-
A 16-bit image has a maximum grey level of 65535
Select the saturation option and the saturated pixels are displayed in red:
Original display
UVITEC – Cambridge
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Inverted image - Negative image
(black spot, light background)
Same image with the saturated pixels
indicated in red
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