UVITEC Cambridge FIREREADER MAX User & Service Manual page 132

The dashboard details the noise subtraction parameters:
FireReader 1D has several functions to minimize image background.
The rolling ball approach
The linear approach
R
OLLING BALL
The rolling ball method is named for a hypothetical ball that rolls along underneath the lane
profile, removing the intensity levels along the length of the lane.
The rolling ball subtraction method removes background levels along the length of the lanes
according to the size of a virtual ball. The ball is rolled under each profile of the image so its
movement varies along the image. The ball follows the profile trace, touching fewer points
along the trace and removing the lower part of the points which defined the subtracted
background.
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