UVITEC Cambridge FIREREADER MAX User & Service Manual page 90

UVITEC – Cambridge
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Some images has a 12, 14 or 16-bit format and Windows®
can only display 8-bit images (256 grey levels).
Due to this limitation, the FireReader 1D software handles
two images:
A "memory" image corresponding to the 12, 14 or 16-bit
format (4 096, 16 384 or 65 536 grey levels)
A "display image" corresponding to the image displayed on
the screen (256 grey levels)
The easiest way to calculate the "display image" would be
to translate the full grey scale each time an image is
acquired: the x grey levels values of the "memory" image
corresponds to 256 values in the displayed image. In that
case, it won't be possible to visualise faint spots on a dark
image.
FireReader 1D offers the possibility to select the grey level
range to translate for the display image calculation. All the
grey levels under the "Min value" defined will be converted
to 0 (Black) in the displayed image. All the grey levels
upper the "Max Value" defined will be set to 255 (White) in
the displayed image. The grey levels between those two
limits will be converted in an intermediate grey level value
following a linear rule.
For both values, you can:
• Edit the value in the corresponding field
• Select the value by dragging and dropping the arrow
• Click on the "optimum display" button: FireReader 1D
will then calculate the ideal values to be selected
according to the parameters defined
Default optimum display
Note: The optimum display has no impact on the
analysis. Only the display of the image is modified.
Inverse or display pseudo color
Click on the "Inverse" icon to inverse the grey level of the
image. This makes a negative image
Optimum display
enhancement
The image appears
brighter. The faint bands
are more visible.