Waters 2690 Quick Start Manual page 54

Figure 6-5 outlines how to change from a buffered solvent to a totally organic solvent in a
sparge-based Separations Module.
Note: All flow chart references refer to sections or figures in the Waters 2690 Separations
Module Operator's Guide.
Reservoir Containing
Attention:
STOP
intermediate solvent such as water when you change from buffers to
high-organic-content solvents. Refer to Section D.3, Solvent Miscibility, for
information on solvent miscibility
Figure 6-5 Changing from a Buffered Solvent to an Organic Solvent
54 Recommended Preparation Procedures
Start
Remove the Solvent
Line from the
the Buffered Solvent
Using a Flow Rate of
2.000
mL/min,
Perform a Wet Prime
Until Air Begins to
Enter the Line
(See Section 4.4.2)
Place the Previously
Removed Solvent
Line into Filtered
HPLC-Grade Water
and Perform a Wet
2
Prime for min at
5
mL/min
(See Section 4.4.2)
To avoid having salts precipitate in the Separations Module, use an
(Sparge-Based Separations Module)
Purge the Injector
for a Minimum of 6
Loop Volumes
(See "Purging the
Injector" in
Section 4.4.2)
Remove the Solvent
Line from the
HPLC-Grade Water
Fill a Clean Solvent
Reservoir with the
Organic Solvent and
Place the Solvent
Line in
the Reservoir
Perform the Steps
Figure 6-1
in