Waters 2690 Quick Start Manual page 52

Figure 6-3 outlines how to prime, equilibrate, and purge a sparge-based Separations
Module that has been powered off or is idle with no solvent flow.
Note: All flow chart references refer to sections in the Waters 2690 Separations Module
Operator's Guide.
Figure 6-3 Preparing an Idle or Powered-Off Separations Module for Operation
52 Recommended Preparation Procedures
Start
Fill the Solvent
Reservoirs with
Solvent Filtered and
Degassed by
Vacuum Filtration
(See Section 2.6.3)
Sparge all
Reservoirs at an
Initial Sparge Rate
100%
of for at
Least 15 min. Set
an Idle Sparge Rate
30 50%
of to
(See Section 4.4.4)
Perform a Wet
Prime (for One of
2
the Solvents) for
5.00
min. at
mL/min. (See
(Sparge-Based Unit)
Repeat the Wet
Prime (at the Same
Rate and Time) for
Each Solvent
To Make a Gradient
Run (for Example,
A:B), Set the A and
B Composition
Fields in the Status
50%
Screen to ,
Then Wet Prime for
1 5.00
min at
mL/min
(See Section 4.4.2)
Equilibrate for a
Minimum of 10
Column Volumes
(See Section 4.4.5)
Purge the Injector
for a Minimum of 6
Loop Volumes
(See "Purging the
Injector" in
Section 4.4.2)