Method Parameter Settings - Agilent Technologies 1290 Infinity User Manual

Method Parameter Settings

The Binary Pump method setup parameters are in eight sections:
• Flow
• Solvents A and B
• Stoptime
• Posttime
• Pressure Limits
• Timetable
• Advanced
• External Contacts
Table 4 Method parameters
Parameter Limits
Flow 0.00 – 5.00 mL/min
in steps of 0.001.
Recommended flow
range: 0.05 –
5.00 mL/min .
Solvents A and B
Stoptime 0.01 – 99999 min or
As Injector/No Limit
(an infinite run time).
Posttime 0.01 – 99999 min or
Off (0.0 min ).
Agilent 1290 Infinity Binary Pump User Manual
Setting up the Pump with the Instrument Control Interface
Description
The flow is the rate of movement of eluent along the column. It is
important that the flow rate is kept constant to ensure precise retention
time, and peak measurements. Variations in flow rate can occur as a
result of the partial failure of the pumping system, air in the pumping
system, a change in the mobile phase viscosity or a temperature change.
For each channel, you can select which of the two solvents to deliver. You
can set the percentage of solvent B to any value from 0 through 100 % .
Solvent A always delivers the remaining volume: 100 - %B. The solvent B
check boxes allow you to turn the solvent B channels on (checked) or off
(cleared).
When the Use solvent types check box in the Compressibility section is
checked (see "Advanced Settings"
and select either a Generic solvent or a calibrated Solvent.
The stoptime sets a time limit for your analysis. After the stoptime, all
gradients are stopped and the pump parameters return to their initial
values. The pump can be used as a stoptime master for the complete
analytical system. The pump also stops the detectors if they have a No
Limit stoptime setting. If no limit is given, a method will have to be
stopped manually.
Your instrument remains in a not ready state during the posttime to delay
the start of the next analysis. You can use the Posttime to allow your
column to equilibrate after changes in solvent composition (for example
after gradient elution).
Using the Pump
on page 67), you click the down arrow
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