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Setting up the Pump with the Instrument Control Interface
Overview
Parameters described in following sections is offered by the instrument
control interface and can usually be accessed through Agilent instrument
control software. For details, please refer to manuals and online help of
respective user interfaces.
Setup of Basic Pump Parameters
The most important parameters of the pump are listed in
Table 3
Basic pump parameters
Parameter
Limits
• Flow
0.001 – 5 mL/min
• Stop Time
0.01 min - no limit
• Post Time
off - 99999 min
• Pressure
Max: 0 – 600 bar
Limits
Min: 0 – 600 bar
• Solvent A
0 – 100 %
• Solvent B
off - 100 %
• Solvent
H
O, ACN, MeOH, IPA
2
type
2
CO
pre-compressed
1260 SFC Binary Pump
Setting up the Pump with the Instrument Control Interface
Description
Total flow rate of the pump.
The stop time of the pump usually controls the run time of the whole LC system.
Use no limit to stop the run manually (useful for method development).
Time between the end of a run and the start of the next. Used for column
equilibration after a gradient.
Max must be bigger than Min! Set max pressure to the maximum operating
pressure of your column. A min pressure setting of e.g. 10 bar will turn off your
pump automatically when running out of solvent. A smarter way, however, is to
use the bottle fillings function (see
Although channel A can be set to 0 %, it cannot be turned off. This channel
should be used for the aqueous phase (water).
The percentage of channel B is automatically complemented by channel A to
give 100 %.
Select the solvent you are using in the respective solvent channel from the
drop-down list. In case your solvent is not listed, perform a solvent
compressibility calibration.
Using the Pump
Table 3
"Bottle Filling"
on page 44).
3
on page 41.
41
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