Hints For Successful Use Of The Nano Pump - Agilent Technologies 1200 Series User Manual

Hints for Successful Use of the Nano Pump

• Flush the pump extensively in the purge mode. It is recommended to do this
• Place the aqueous solvent on channel A and the organic solvent on
• Place solvent cabinet with the solvent bottles always on top (or at a higher
• The system pressure must be higher than 20 Bar at the pump outlet to
• In micro mode abnormally high column flow variations are an indication of
• Prevent blocking of solvent inlet filters (never use the pump without solvent
1200 Series Nano Pump User Manual
for 4 min each, first with 100% A and then 100% B. Small residual bubbles
are removed by pumping in micro mode with the column installed.
channel B. The default compressibility and solvent flow sensor calibration
settings are set accordingly. Always use the correct calibration values.
level) of the nano pump.
achieve stable flow rates.
small particles within the system, partially blocked filters or capillaries.
inlet filter). Growth of algae must be avoided, either by using acidic (pH=3)
media and/or changing solvent every day.
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