Glowell Test; Glowell Results Analysis - Biotek Cytation 5 Instructions For Use Manual

Glowell Test

Materials
• Glowell, PN GLO-466, formerly available from LUX BioTechology, Ltd.
(www.luxbiotech.com)
• Glowell Adapter Plate, available from BioTek, PN 7160006
• Filter cube PN 8040553, LUM Filter Block,
EX position 1 or 2 and a hole in the corresponding EM position.
• Gen5 protocols described starting on page 88
Procedure
1. If you have not already done so, insert the Glowell ("window" side up) into
well D8 of the Adapter Plate.
2. Create an experiment based on the
Cytation 5 M-LumTest_Glowell.prt
3. Calculate and evaluate the results as described on page 87.
Plate Layout
1 2 3
A
B
C
D
E
F
G
H

Glowell Results Analysis

1. Locate these items on the Glowell's Calibration Certificate: Calibration Date,
Radiant Flux (pW), Measurement Uncertainty of the Radiant Flux.
2. Calculate the number of days between the Calibration Date and the date the
test was performed.
3. Correct the Glowell's Radiant Flux value for deterioration over time:
Radiant Flux * (1 – 0.000333 * number of days since calibration)
4. Convert the Corrected Radiant Flux value to attomoles:
(Corrected Radiant Flux / 0.021) * 1800
Cytation 5
or
Cytation 5 F-Lum-Test_Glowell.prt
protocol, and read the plate.
4 5 6 7
Luminescence Test |
a filter cube with a plug in
8 9 10
BUF BUF
BUF BUF
BUF BUF
BUF BUF
Glowell
BUF BUF
BUF BUF
BUF BUF
BUF BUF
87
or
11 12
BUF
BUF
BUF
BUF
BUF
BUF
BUF
BUF