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30
| Getting Started
Components
Tubing, syringe fittings
Injector tips
Injector body
Priming plate and trough
Valve diaphragms
Valve body
Syringe barrel
Priming the Injector System
Before running a dispense assay, prime the system with the reagent or dispensing
fluid. In addition, tip priming can be performed at the start of an assay and,
sometimes, just before each dispense to a well. The tip prime compensates for any
fluid loss at the injector tip due to evaporation since the last dispense. All priming
activities are controlled via Gen5.
If the injector system is not primed adequately, air bubbles can get
trapped in the system and affect injection volumes. Air bubbles in the
system can also result in fluid spraying or scattering inside the reader.
Both types of primes require a fluid reservoir to be present on the microplate carrier.
• The priming plate is placed on the microplate carrier for a Prime operation (to
prime the dispense system with fluid).
• The tip priming trough is placed in the rear pocket of the carrier, and is used for
performing the Tip Prime before dispensing. The trough holds up to 1.5 mL of
liquid and must be periodically emptied and cleaned by the user.
Do not perform tip priming when using tall plates. Generally, plates
with fewer than 96 wells are too tall for error-free tip priming; and, tip
priming is rarely required for these larger-volume plates.
The priming tray should be empty before priming and contain fluid after
priming.
Imaging System
Instruments with the imaging module can perform image reads, view and capture
images in manual mode, and save the images for later analysis. The imaging module
comprises up to four LED cubes and four imaging filter cubes, up to six objectives,
and a CCD digital camera, which captures images directly through the selected
objective and filter cube assembly. The imaging module supports two modes,
manual mode and experiment mode.
Material Composition
PTFE (polytetrafluoroethylene)
315 stainless steel
PVC (polyvinyl chloride)
Polyproylene
Ethylene propylene (EPDM)
PEEK (polyether ether ketone)
Borosilicate glass
BioTek Instruments, Inc.
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Troubleshooting
