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| Getting Started
and the values for Horizontal and Vertical offset from the center of the well
to your read step.
4. Select
to turn Auto Exposure back on. Gen5 will auto-expose your
Auto
image, retaining the well offset imported from manual mode.
You can also enter the values for Horizontal and Vertical offset from
center of well if you had determined them during previous testing.
Dispense Module Control
This section applies only to models with injectors.
Gen5 is used to perform several dispense functions, such as initialize, dispense,
prime, and purge. The Prime and Purge functions are introduced here. See the Gen5
Help system for more information.
Priming and purging routines are used to clean the fluid paths.
Prime
Before running an experiment with a dispense step, prime the system with the fluid
to be used.
1. Place the priming plate on the carrier.
2. Fill the supply bottle with a sufficient volume of the fluid to be used for the
prime and the assay. Insert the appropriate inlet tube into the bottle.
3. In Gen5, select
the
tab.
Prime
4. Select the Dispenser number (1 or 2) associated with the supply bottle.
5. Enter the Volume to be used for the prime. The minimum recommended
prime volume is 2000 µL.
6. Select a prime Rate, in µL/second.
7. Click
Prime
8. When finished, carefully remove the priming plate from the carrier and
empty it.
If the priming plate is empty, the prime volume was too low.
Purge
To save reagent, Gen5 provides the option to purge fluid from the system back into
the supply bottle.
System > Instrument Control > Cytation 5
to start the process.
and click
BioTek Instruments, Inc.
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Table of Contents
Troubleshooting
