Biotek Cytation 5 Instructions For Use Manual page 92

80 | Instrument Testing
Parameter
Read Speed:
Read Height:
Cytation 5_FP.prt
This procedure contains one Read step using filters with Fluorescence
Polarization enabled, inside a Plate Mode block.
Parameter
Detection Method:
Read Type:
Plate Type:
Shake Step:
Delay Step:
Synchronized Mode:
Read Wells:
Filter Sets:
Filters:
Optics Position:
Gain:
Read Speed:
Read Height:
Cytation 5_TRF.prt
Parameter
Plate Type:
Delay Step:
Read Step 1
Detection Method:
Default Setting
Normal
Delay after plate movement: 350 msec
Measurements per data point: 50
Lamp Energy: Low
Dynamic Range: Standard
7.00 mm
Default Setting
Fluorescence polarization
Endpoint
Costar 96 black opaque (#3915)
Linear for 15 seconds
5 seconds after shake
Plate Mode with Timing Control
A6–H8
1 (filter cube)
EX 485/20 nm, EM 528/20 nm
Top 510 nm
Auto, Scale to high Wells, A8, 10000
Normal
Delay after plate movement: 350 msec
Measurements per data point: 60
Lamp Energy: Low
7.0 mm
Default Setting
Costar 96-well white opaque
3 minutes
Time-resolved fluorescence
BioTek Instruments, Inc.