Autofocus Reliability Tests - Biotek Cytation 5 Instructions For Use Manual

Parameter
Vibration Detection Options:
Horizontal offset from center of well:
Vertical offset from center of well:
Single image per well:
Delay after plate movement:

AutoFocus Reliability Tests

Cytation 5 AF_Reliability_Meiji 2.5X.prt, Cytation 5 AF_Reliability_Zeiss
2.5X.prt
Parameter
Detection Method:
Read Type:
Plate Type:
Kinetic Step:
Objective:
Read Wells:
Color Channel:
Exposure:
Auto-Focus Options:
Auto-Exposure Options:
Vibration Detection Options:
Horizontal offset from center of
well:
Vertical offset from center of well:
Cytation 5
Default Setting
CV Threshold: 0.01
Images to average: 1
0
0
Selected
300 msec
Default Setting
Imaging
Endpoint
1222520_AF (imaging qualification plate)
Run Time: 19:59, Interval: 12 seconds, Reads:
100
Meiji 2.5X/Zeiss 2.5X
F7
DAPI 377, 447
Auto
Focus method: Auto focus with optional scan
Minimum focus metric ratio: 3
Scan distance: 600
Scan increment: 30
Minimum focus delta: 8
% of capture exposure for focus: 75
Offset from bottom of well: -50
Target exposure %: 75
Skip %: 0.1
Integration threshold: 100
CV Threshold: 0.01
Images to average: 5
0
0
Imaging Tests | 63