Glowell Test - Biotek Synergy H1 Instructions For Use Manual

Glowell Test

Materials
•
Glowell, PN GLO-466, formerly available from LUX BioTechnology, Ltd.
(www.luxbiotech.com)
•
Glowell Adapter Plate, available from BioTek, PN 7160006
•
Gen5 protocol (see page 68)
Procedure
1. Insert the Glowell ("window" side up) into well D8 of the Adapter Plate.
Create the Gen5 protocol as described on page 68.
2.
Create an experiment based on the F-LumTest_Glowell.prt (for filter-based
3.
luminescence) or M-LumTest_Glowell.prt (for monochromator-based
luminescence) protocol.
Calculate and evaluate results as described under
4.
Results Analysis
A manual ATP correlation process determined that 0.021 pW Radiant
Flux is equivalent to approximately 1800 attomoles of ATP.
1. Locate these items on the Glowell's Calibration Certificate:
Date
2. Calculate the number of days between the Calibration Date and the date
the test was performed.
3. Correct the Glowell's Radiant Flux value for deterioration over time:
Radiant Flux * e^(-0.0001536*number of days since calibration)
4. Convert the Corrected Radiant Flux value to attomoles (see Note above):
(Corrected Radiant Flux / 0.021) * 1800
Calculate an error factor for the Corrected Radiant Flux (amol):
5.
(Corrected Radiant Flux in amol * Measurement Uncertainty) / 100
6. Calculate the min/max criteria for the Corrected Radiant Flux (amol):
MIN: Corrected Radiant Flux in amol – Error Factor
MAX: Corrected Radiant Flux in amol + Error Factor
7. Calculate the Signal-to-Noise Ratio:
Measurement value of the Glowell – Mean of Column 9
8. Calculate the Detection Limit:
Corrected Radiant Flux in amol / Signal-to-Noise Ratio
Synergy H1
, Radiant Flux (pW) ,
3 x Standard Deviation of Column 9
Results Analysis
Measurement Uncertainty of the Radiant Flux
Luminescence Test |
.
Calibration
67
.