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| Instrument Testing
Time-Resolved Fluorescence (TRF) Test
• Pipette 200 µL of deionized water into wells A6–C6.
• If you have not already done so, shake the vial of 20 pM europium suspension
vigorously for 30 seconds prior to pipetting. Alternatively, sonicate or vortex
the vial.
• Pipette 200 µL of the 20 pM europium suspension (Eu) into well A8.
1
2
3
A
B
C
D
E
F
G
H
Results Analysis
Corners Test
1. Calculate the Mean of the 12 wells containing the 3.3 nM SF test solution
(A1–A3, A10–A12, H1–H3, and H10–H12).
2. Calculate the Standard Deviation for the same 12 wells.
3. Calculate the %CV:
The %CV must be
< 3.0
Sensitivity Test
1. Calculate the Mean and Standard Deviation of the 16 reads for each of the
buffer wells (C9, D9, E9).
2. Among the three buffer wells, find the Median Standard Deviation and
corresponding Mean.
3. Calculate the Mean for the 16 reads of the SF Concentration well (D7).
4. Calculate the Signal-to-Noise Ratio (SNR) using the Mean SF Concentration,
Buffer Median STD with its corresponding Buffer Mean:
(SF Mean – Buffer Mean)/(3 * Buffer STD)
5. Calculate the Detection Limit, in pM, using the known concentration value of
SF and the Calculated
4
5
6
7
DI
DI
DI
(Standard Deviation / Mean) * 100
to pass.
SNR: 1000/SNR
8
9
10
Eu
BioTek Instruments, Inc.
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12
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Troubleshooting
