BioTek Cytation 5 Manuals

Manuals and User Guides for BioTek Cytation 5. We have 1 BioTek Cytation 5 manual available for free PDF download: Instructions For Use Manual

BioTek Cytation 5 Instructions For Use Manual (123 pages)

Cell Imaging Multi-Mode Reader

Brand: BioTek | Category: Card Reader | Size: 1 MB

Notices
2
Instructions for Use Requirements
4
Intended Use Statement
4
Quality Control
4
Warnings
5
Hazards
5
Precautions
6
Electromagnetic Interference and Susceptibility
9
User Safety
10
Safety Symbols
11
Installation
14
Package Contents
14
Unpack and Inspect the Reader
15
Unpack and Inspect the Dispenser
16
Unpack and Inspect the Gas Controller
16
Select an Appropriate Location
16
Installing Instruments with the Isolation Table
17
Remove the Shipping Hardware
18
Install the Power Supply
19
Install the Gas Controller
20
Unpack and Install the Joystick
20
Install the Dispenser
20
Connect the Host Computer
22
Install Gen5
22
Turn on the Reader
22
Establish Communications
22
Install the Imager Module
23
Communication Errors
23
Firewire Video Card: Computer and Camera Setup
23
Desktop Computer: Install the Firewire Card
24
Laptop Computer: Install the Firewire Card
25
Install the Firewire Driver
26
Establish Communication with the Camera
27
Troubleshooting Communication with the Camera
27
Set up Gen5 for Imaging
28
Setting the Objective, LED Cube, and Imaging Filter Cube Configuration
29
Install the Objectives
29
Install the LED Cubes and Imaging Filter Cubes
30
Run Auto Calibration
32
Troubleshooting Auto Calibration
32
Configure the Dispensers in Gen5
33
Test the Dispenser
34
Run a System Test
35
Repackaging and Shipping Instructions
36
Getting Started
38
Overview
38
External Components
38
Internal Components
39
Filter Cube
40
Injector System
40
Define the Filters
40
Configuring the System for Luminescence Measurements
40
Dispense Module
41
Dispense Module Components and Materials Composition
41
Priming the Injector System
42
Imaging System
42
Camera
43
Objectives
43
Imaging Modes
43
Change the Virtual Memory Settings
43
Shaking System
44
Carrier Shake Definitions
45
Maximum Shaking Amplitude Based on Assay Volume and Plate Type
45
Gen5 Software
46
Define the Fluorescence Filter Cube
47
Protocols and Experiments
47
Imaging Module: Manual Mode
48
Focusing the Camera
48
Imaging Module: Experiment Mode
50
Analyze Captured and Saved Images
50
Perform an Image Read
50
Using the Slide Holder
51
Manual Mode
51
Experiment Mode
51
Dispense Module Control
52
Prime
52
Purge
52
Preventive Maintenance
56
Warnings and Precautions
56
Daily Cleaning for the Dispense Module
56
Clean Exposed Surfaces
57
Inspect/Clean Mirrors
58
Materials
59
Procedure
59
Inspect/Clean Excitation and Emission Filters
60
Clean the Objectives
61
Flush/Purge the Fluid Path
62
Empty/Clean the Tip Priming Trough
63
Clean the Priming Plate
64
Clean the Dispense Tubes and Injectors
64
Required Materials
64
Remove the Dispense Tubes and Injector Holders
64
Instrument Testing
68
System Test
68
Absorbance Plate Test
68
Setup
68
Imaging Tests
70
Carrier Level Test
71
Autofocus Test
71
Analyze the Results
71
Troubleshooting
73
Imaging Protocols
73
Carrier Level Tests
73
Autofocus Reliability Tests
75
Absorbance Liquid Test
77
Fluorescence Liquid Tests
79
Corners/Sensitivity/Linearity Tests
80
Fluorescence Polarization (FP) Test
81
Time-Resolved Fluorescence (TRF) Test
81
Test Solutions
82
Pipette Maps
84
Results Analysis
86
Corners Test
86
Sensitivity Test
86
Linearity Test
87
Gen5 Protocol Reading Parameters
89
Luminescence Test
96
Harta Plate Test
96
Plate Layout
97
Harta Plate Results Analysis
98
Glowell Test
99
Glowell Results Analysis
99
Protocol Reading Parameters
100
Alpha Detection Test
104
Pipette Map
105
Crosstalk Test
105
Troubleshooting Alpha Tests
106
Dispense Module Tests
107
Alternate Test Solutions
108
Using Biotek’s Blue and Yellow Concentrate Dye Solutions
108
Using FD&C Blue and Yellow Dye Powder
108
Gen5 Test Protocols for Models with Absorbance Capabilities
110
Define the Procedure
110
Gen5 Procedure Steps
110
Add Data Reduction Steps
111
General Specifications
114
Dispense/Read Specifications
116
Absorbance Specifications
117
Fluorescence Specifications (Mono-Based)
119
Fluorescence Specifications (Filter-Based)
120
Luminescence Specifications
121
Imaging Specifications
121

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