Fluorescence Polarization (Fp) Test; Time-Resolved Fluorescence (Trf) Test - Biotek Cytation 5 Instructions For Use Manual

If testing both Top and Bottom optics (mono-based fluorescence only):
•
new, clean 96-well glass-bottom Greiner SensoPlate (Mfr. #655892); or a clean
Hellma Quartz 96-well titration plate (Mfr. #730.009.QG); or equivalent
• If testing the Top optics only:
as Corning Costar #3915, or equivalent
• Excitation filter 485/20 nm installed
• Emission filter 528/20 nm installed
• 510-nm dichroic mirror installed

Fluorescence Polarization (FP) Test

• A new, clean, 96-well solid black microplate, such as Corning Costar #3915. A
Greiner SensoPlate can also be used.
The FP Test can be performed in conjunction with the top
Corners/Sensitivity/Linearity Tests, in the same microplate.
• The recommended test solutions are available from Invitrogen Corporation in
their "FP One-Step Reference Kit" (PN P3088) or from BioTek (PN 7160014).
This kit includes:
 (Green) Polarization Reference Buffer, 15 mL
 Green Low Polarization Reference, 4 mL
 Green High Polarization Reference, 4 mL
The kit also includes two red polarization solutions; these are not used.
• Excitation filter 485/20 nm installed
• Emission filter 528/20 nm installed
• 510-nm dichroic mirror and polarizers installed

Time-Resolved Fluorescence (TRF) Test

• 15-mL conical-bottom, polypropylene sample tube
• Excitation filter 360/40 nm installed
• Emission filter 620/40 nm installed
• 400-nm dichroic mirror installed
• A new, clean 96-well solid white microplate, such as Corning Costar #3912
• The recommended test solution (FluoSpheres carboxylate-modified
microspheres, 0.2 µm europium luminescent, 2 μL) is available from Invitrogen
Corporation (PN F20881) or from BioTek (PN 7160011)
Cytation 5
Fluorescence Liquid Tests |
A new, clean 96-well solid black microplate, such
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