Imaging Tests - Biotek Cytation 5 Instructions For Use Manual

58 | Instrument Testing
9. Review all the values you entered. Click
The information you just entered is available on Gen5 each time the Absorbance Plate
Test is performed. It may need to be modified after the annual recertification of your
test plate.
Procedure
1. From the Gen5 main screen, select
. If prompted, select the desired Test Plate and click
> Run
2. When the Absorbance Test Plate Options dialog appears, select
Peak Wavelength Test
3. Highlight the wavelength(s) to be included in this test.
Select only those wavelengths most appropriate for your use of the
reader.
4. (Optional) Enter any Comments.
5. Click
Start Test
6. Place the Test Plate in the microplate carrier so that well A1 is in the right-
rear corner of the carrier.
7. Click to run the test.
OK
8. When the test is completed, the results report appears. Scroll through the
report; every result should show "PASS".

Imaging Tests

These procedures test the level position of the carrier and the reliability of the Auto
Focus feature.
Required Materials
• Imaging qualification plate, PN 1222520
• 10X or lower objective
• DAPI 377/447 cube (PN 1225100)
• Gen5 protocols described beginning on page 61
Cytation 5 <x>X_CarrierLevel.prt
•
Cytation 5 AF_Reliability_<x>X.prt
•
System > Diagnostics > Test Plates
if it is not already selected.
.
to save the data.
OK
.
OK
BioTek Instruments, Inc.
Perform