Biotek Cytation 5 Instructions For Use Manual page 83

Fluorescence Polarization (FP) Test
The recommended test solutions are available from Invitrogen Corporation or from
BioTek. They do not require additional preparation.
Time-Resolved Fluorescence (TRF) Test
The recommended test solutions are available from Invitrogen Corporation or from
BioTek.
• Shake the FluoSpheres container vigorously for 30 seconds prior to pipetting.
Alternatively, sonicate or vortex the container.
• Mix 10 µL of FluoSpheres with 10 mL of deionized water, in a 15 mL conical-
bottom, polypropylene sample tube. This yields a 20 nM equivalent suspension.
• Shake the vial vigorously for 30 seconds prior to pipetting. Alternatively,
sonicate or vortex the container.
• Mix 10 µL of 20 nM suspension with 10 mL of deionized water, in a 15 mL
conical-bottom, polypropylene sample tube. This yields a 20 pM equivalent
suspension.
• Refrigerate any unused portions of the FluoSpheres. The temperature must be
between +2°C to +6°C.
The prepared TRF plate can be kept for a maximum of seven days, if
covered and stored in the dark between +2°C to +6°C.
Allow the plate to sit at room temperature for approximately 15 minutes
prior to use.
Procedure
1. If you have not already done so, create the Gen5 protocols described starting
on page 77.
2. If you have not already done so, prepare the solutions for the tests you plan
to perform. See
Refer to the pipette maps, next, for the remaining steps.
3. Perform the Corners/Sensitivity/Linearity tests using the Top optics of the
filter-based fluorescence system:
• Pipette the test solutions into a clean 96-well microplate.
• Create an experiment based on the
the plate, and then save the experiment.
Cytation 5
Fluorescence Test Solutions
Cytation 5_FI_T_SF.prt
Fluorescence Liquid Tests |
on page 70.
protocol. Read
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