Instrument Testing; System Test; Absorbance Plate Test; Setup - Biotek Cytation 5 Instructions For Use Manual

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| Instrument Testing

System Test

Each time the Cytation 5 is turned on, it automatically performs a series of tests on
the reader's motors, lamp(s), the PMT(s), and various subsystems. If all tests pass, the
microplate carrier is ejected and the green LED on the carrier switch remains on.
If any test results do not meet the internally coded Failure Mode Effects Analysis
(FMEA) criteria established by BioTek, the reader beeps repeatedly and the red LED
on the carrier switch flashes. If this occurs, press the carrier eject button to stop the
beeping. If necessary, initiate another system test using Gen5 to try to retrieve an
error code from the reader.
1. Turn on the reader and launch Gen5.
2. If your assays use incubation, we recommend enabling Temperature Control
and allowing the incubator to reach its set point before running the system
test. To access this feature, from the Gen5 main screen, select
Instrument Control
3. Select
System > Diagnostics > Run System Test
4. When the test is complete, a dialog appears, requesting additional
information. Enter your user name and other information (if desired) and
then click
OK
5. The results report appears. It shows either "SYSTEM TEST PASS" or
"SYSTEM TEST FAIL *** ERROR (error code) DETECTED."
6. Print the report if desired.
7. If the test failed, look up the error code in
determine its cause. If the cause is something you can fix, turn off the reader,
fix the problem, and then turn the reader back on and retry the test. If the test
continues to fail, or if the cause is not something you can fix, contact BioTek's
Technical Assistance Center.

Absorbance Plate Test

This test uses BioTek's Absorbance Test Plate (PN 7260522) to confirm mechanical
alignment; optical density accuracy, linearity, and repeatability; and wavelength
accuracy. The Absorbance Plate Test compares the reader's optical density and
wavelength measurements to NIST-traceable values. The Absorbance OD Standards
section contains NIST-traceable standard OD values for the filters at several different
wavelengths. We recommend testing at six wavelengths—those at or close to the
wavelengths used in your assays.

Setup

1. Obtain the certificates that came with the Test Plate.
2. Start Gen5 and from the main screen select
Plates > Add/Modify Plates
and click the
.
.
tab.
Pre-Heating
.
the Cytation 5 Operator's Manual
System > Diagnostics > Test
BioTek Instruments, Inc.
System >
to