Biotek Cytation 5 Instructions For Use Manual page 90

78 | Instrument Testing
The Plate Type setting in each Gen5 protocol should match the plate you
are actually using.
Cytation 5_FI_T_SF.prt
This procedure contains two Read steps using filters to test the top
optics: one for the Corners Test and one for the Sensitivity/Linearity Test.
Parameter
Plate Type:
Read Step 1
Detection Method:
Read Type:
Kinetic Loop
Step Label:
Read Well:
Filter Set:
Filters:
Optics Position:
Gain:
Read Speed:
Read Height:
Read Step 2
Detection Method:
Read Type:
Kinetic Loop
Step Label:
Read Wells:
Filter Set:
Default Setting
Costar 96 black opaque (#3915)
Fluorescence
Endpoint
Run Time: 00:00:45
Interval: 00:00:03
Reads: 16
"Sensitivity Read"
D7
1 (Green)
EX 485/20 nm, EM 528/20 nm
Top 510 nm
Auto, Scale to High Wells, D7, 30000
Normal
Delay after plate movement: 350 msec
Measurements per data point: 50
Lamp Energy: Low
Dynamic Range: Standard
7.00 mm
Fluorescence
Endpoint
Run Time: 00:01:35
Interval: 00:00:06
Reads: 16
"Sensitivity Read Buffer"
C9..E9
1 (Green)
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