Luminescence Test; Harta Plate Test - Biotek Cytation 5 Instructions For Use Manual

84 | Instrument Testing
Parameter
Read Speed:
Read Height (for top optics):

Luminescence Test

BioTek provides two methods for verifying the performance of luminescence reads.
One method measures a Harta Luminometer Reference Microplate, which is an LED-
based test plate. Contact BioTek to purchase a plate, or go to
www.hartainstruments.com for more information. The other method measures a
LUX Biotechnology, Ltd., Glowell unit, which is a small, sealed cylinder with a
gaseous tritium light source.
Before using the
Cytation 5 F-LumTest_Harta.prt
LumTest_Glowell.prt
below.

Harta Plate Test

Materials
• Harta Luminometer Reference Microplate, PN 8030015
• Harta Plate Adapter, PN 1222205
• Filter cube PN 8040553, LUM Filter Block
EX position 1 or 2 and a hole in the corresponding EM position
• Gen5 protocol described started on page 88
Default Setting
Normal
Delay after plate movement: 100 msec
Measurements per data point: 50
Lamp Energy: Low
Dynamic Range: Standard
7.00 mm
protocols described in this section, create the filter set shown
or
Cytation 5 F-
a filter cube with a plug in
or
BioTek Instruments, Inc.