Imaging Module: Manual Mode; Focusing The Camera - Biotek Cytation 5 Instructions For Use Manual

36 | Getting Started
For imaging reads, you must also define the Bottom Elevation parameter
for the plate or slide. If the relevant measurements are not defined, Gen5
will not allow the objective to move closer than 1 mm from the bottom of
the carrier, thus disabling Cytation 5's focus capabilities.
4. Add steps to the procedure for shaking or heating the plate, dispensing fluid,
reading the plate, and more. Click
supports the defined steps, and then click
Optionally, perform the next steps to analyze and report the results:
5. Open the Plate Layout dialog and assign blanks, samples, controls, and/or
standards to the plate.
6. Open the Data Reduction dialog to add data reduction steps. Categories
include Transformation, Well Analysis, Curve Analysis, Cutoff, and
Validation.
7. Create a report or export template via the Report/Export Builders.
8. Select
File > Save
These instructions briefly describe how to create an experiment and then read a plate
in Gen5. See the Gen5 Help system for complete instructions.
1. Open a new experiment.
2. Select the desired protocol and click
3. Select a plate in the menu tree and read it.
4. When the read is complete, measurement values appear in Gen5. Select the
desired data set from the Data list.
5. Select
File > Save

Imaging Module: Manual Mode

Applies only to models with the imaging module.
The following sections
manual mode. See the Gen5 Help for more complete instructions and descriptions of
these features.

Focusing the Camera

1. From the Task Manager, select
Alternatively, from the main Gen5 screen, click
2. Click
Find Image
image. If the displayed image requires additional fine-tuning:
• Click
Auto Expose
see an image.
Validate
and give the file an identifying name.
OK
and give the file an identifying name.
describe how to use the Gen5 imaging module in
briefly
Read Now > Run Imager Manual Mode
. Gen5 automatically sets the exposure and focuses on the
, or manually adjust the Exposure settings until you
to verify that the reader
.
OK
.
.
BioTek Instruments, Inc.
.