Biotek Cytation 5 Instructions For Use Manual page 76

64 | Instrument Testing
Parameter
Single image per well
Delay after plate movement:
Cytation 5 AF_Reliability_4X.prt
Parameter
Detection Method:
Read Type:
Plate Type:
Kinetic Step:
Objective:
Read Wells:
Color Channel:
Exposure:
Auto-Focus Options:
Auto-Exposure Options:
Vibration Detection Options:
Horizontal offset from center of
well:
Vertical offset from center of well:
Single image per well
Delay after plate movement:
Cytation 5 AF_Reliability_10X.prt
Parameter
Detection Method:
Read Type:
Default Setting
Enabled
0 msec
Default Setting
Imaging
Endpoint
1222520_AF (imaging qualification plate)
Run Time: 19:59, Interval: 12 seconds, Reads:
100
4X
F7
DAPI 377, 447
Auto
Focus method: Auto focus with optional scan
Minimum focus metric ratio: 25
Scan distance: 600
Scan increment: 30
Minimum focus delta: 8
% of capture exposure for focus: 75
Offset from bottom of well: -50
Target exposure %: 75
Skip %: 0.1
Integration threshold: 100
CV Threshold: 0.01
Images to average: 5
0
0
Enabled
0 msec
Default Setting
Imaging
Endpoint
BioTek Instruments, Inc.