Biotek Synergy H1 Instructions For Use Manual
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Biotek Synergy H1 Instructions For Use Manual

Hybrid multi-mode microplate reader for in vitro diagnostic use
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See also: Operator's Manual
Synergy™ H1
Hybrid Multi-Mode Microplate Reader
Instructions for Use
For In Vitro Diagnostic Use
June 2012
© 2012
Part Number 8041005
Revision B
®
BioTek
Instruments, Inc.

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Summary of Contents for Biotek Synergy H1

  • Page 1 Synergy™ H1 Hybrid Multi-Mode Microplate Reader Instructions for Use For In Vitro Diagnostic Use June 2012 © 2012 Part Number 8041005 Revision B ® BioTek Instruments, Inc.
  • Page 2 BioTek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.
  • Page 3 802-655-4740 (outside the U.S.) Fax: 802-654-0638 E-Mail: tac@biotek.com European Coordination Center/Authorized European Representative ® BioTek Instruments GmbH Kocherwaldstrasse 34 D-74177 Bad Friedrichshall Germany Internet: www.biotek.de Phone: +49 (0) 7136 9680 Fax: +49 (0) 7136 968 111 E-Mail: info@biotek.de Synergy H1...
  • Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. The user must evaluate this instrument and PC-based software in conjunction with their specific assay(s).
  • Page 5 If a spill occurs while a program is running, abort the program and turn off the instrument. Wipe up all spills immediately. Do not operate the instrument if internal components have been exposed to fluid. Contact BioTek TAC for assistance. Synergy H1...
  • Page 6 Precautions The following precautions are provided to help avoid damage to the instrument: Caution: Service. The instrument should be serviced by BioTek-authorized personnel. Caution: Spare Parts. Only approved spare parts should be used for maintenance. The use of unapproved spare parts and accessories may result in a loss of warranty and potentially impair instrument performance or cause damage to the instrument.
  • Page 7 Caution: Electromagnetic Compatibility. Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g., unshielded intentional RF sources), because these may interfere with the proper operation. Synergy H1...
  • Page 8 EN 61010-1, “Safety requirement for electrical equipment for measurement, control and laboratory use. Part 1, General requirements.” EN 61010-2-81, “Requirements for automatic and semi-automatic laboratory equipment for analysis and other purposes.” BioTek Instruments, Inc.
  • Page 9 Department of Communications. Le present appareil numerique n'émet pas de bruits radioelectriques depassant les limites applicables aux appareils numerique de la Class A prescrites dans le Reglement sur le brouillage radioelectrique edicte par le ministere des Communications du Canada. Synergy H1...
  • Page 10 Part 1: general requirements” • Canadian Standards Association CAN/CSA C22.2 No. 61010-1 “Safety requirements for electrical equipment for measurement, control and laboratory use; Part 1: general requirements” • EN 61010 standards: see on page viii CE Mark BioTek Instruments, Inc.
  • Page 11 Attenzione, rischio di schiacciare ed intrappolarsi Warning, hot surface Warning, potential biohazards Attention, surface chaude Attention, risques biologiques potentiels Warnen, heiße Oberfläche Warnung! Moegliche biologische Giftstoffe Precaución, superficie caliente Atención, riesgos biológicos Attenzione, superficie calda Attenzione, rischio biologico Synergy H1...
  • Page 12 Recogida selectiva de aparatos eléctricos y electrónicos Dispositivo medico diagnostico in vitro Raccolta separata delle apparecchiature elettriche ed elettroniche Consult instructions for use Consulter la notice d’emploi Gebrauchsanweisung beachten Consultar las instrucciones de Consultare le istruzioni per BioTek Instruments, Inc.
  • Page 13 Installation...

  • Page 14: Package Contents

    | Installation Package Contents Item Part # Synergy H1 Operator’s Manual 8041000 Power supply 76061 Power cord set (specific to installation environment): Europe (Schuko) 75010 USA/International 75011 United Kingdom 75012 Australia/New Zealand 75013 RS-232 serial cable 75034 75108 USB cable with USB Driver Software...
  • Page 15 55 pounds (24.95 kg) Save all packaging materials. If you need to ship the instrument to BioTek for repair or replacement, you must use the original materials. Using other forms of commercially available packaging, or failing to follow the repackaging instructions, may...
  • Page 16 A clean work area is necessary to ensure accurate readings. If you are installing the BioStack for operation with the Synergy H1, be sure to allow enough room for both instruments and the host computer. The BioStack and the reader require a space approximately 42"...
  • Page 17 Never use a plug adapter to connect primary power to the instrument. Use of an adapter disconnects the utility ground, creating a severe shock hazard. Always connect the system power cord directly to an appropriate receptacle with a functional ground. Synergy H1...
  • Page 18 Place the dispense module on top of the gas controller (if used) or the reader. Figure 2 Open the plastic bag containing the injector tubes and tips (labeled as PN 8040541). Remove the clear plastic shrouds from the tubes. BioTek Instruments, Inc.
  • Page 19 Open the door on the front of the reader, and, holding the injector tip holder by the tab, insert the injector tips into the appropriate holes inside the reader Figure 3 Injector holder Figure 3 Synergy H1...
  • Page 20 Carefully pull down the bottom of the syringe until it rests inside the hole in the bracket. • Pass a thumbscrew up through this hole and thread it into the bottom of the syringe. Hold the syringe from rotating while tightening the thumbscrew. Finger-tighten only. BioTek Instruments, Inc.
  • Page 21 Locate the injector tip-cleaning stylus, packaged in a small cylinder. Attach the cylinder to the back of the dispense module for storage. 9: Connect the Host Computer The Synergy H1 is equipped with two communication ports: Serial (RS-232) and USB. Both ports are located on the back of the reader. •...
  • Page 22 | Installation 10: Install Gen5 on the Host Computer The Synergy H1 is controlled by Gen5 software running on a host computer. There is a certain sequence of events that must be followed to ensure that the software is properly installed and configured.
  • Page 23 Properties (e.g., Serial Port (COM5)). • Select Plug & Play A Synergy H1 must be connected to the computer and turned on to appear in the Available Plug & Play Readers list. Click . Gen5 attempts to communicate with the reader. If the Test Comm communication attempt is successful, return to Gen5’s main screen.
  • Page 24 | Installation • Select System > Instrument Control > Synergy H1 • Click the Pre-Heating tab and click • Return to Gen5’s main screen. Models with injectors: Keep Gen5 open and proceed to the next section, Test the Injector System All other models: The installation and setup process is complete.
  • Page 25 Examine the fittings; no leaks should be detected. If leaks are detected, tighten all fittings and repeat the prime. If leaks are still detected, contact BioTek’s Technical Assistance Center.
  • Page 26 • Filter reader shipping bracket (PN 8042187) If you need to ship the Synergy H1 and/or the dispense module to BioTek for service or repair, be sure to use the original packaging materials. Other forms of commercially available packaging are not...
  • Page 27 Getting Started...
  • Page 28 | Getting Started Overview The Synergy H1 is a hybrid multi-mode microplate reader, with a design that allows you to initially purchase only the modules you need and then upgrade later as your requirements expand. Please contact BioTek Customer Care to learn more about your upgrade options.
  • Page 29 Internal Components This section introduces some internal components that may require replacement or cleaning over time. Not all of these components exist in all Synergy H1 models. Filter Cube The Synergy H1 is equipped with a filter cube that contains excitation and emission filters, mirrors, and, if required, polarizing filters.
  • Page 30 BioTek recommended option. Alternatively, you can install different mirrors in the filter cube. Contact BioTek for more information on purchasing additional mirrors and filter cubes. The Synergy H1’s filter cube stores up to two mirrors. There are two possible mirror types: •...
  • Page 31 If a syringe is leaking, it may need to be replaced. Dispense Module Each dispense module is calibrated to perform with its specific Synergy H1 reader. Make sure the same serial number appears on both the dispense module and the reader.
  • Page 32 Valves switch the syringe flow from the inlet tubes to the outlet tubes. Outlet tubes transport fluid from the syringes into the instrument, through the tubing ports on the Synergy H1’s top cover. The outlet tubes are opaque PTFE tubes with threaded fittings on each end.
  • Page 33 Figure 9 Gen5 Software Gen5 software supports all Synergy H1 reader models. Use Gen5 to control the reader and the dispense module (if equipped), perform data reduction and analysis on the measurement values, print or export results, and more. This section provides brief instructions for working with Gen5 to create protocols and experiments and read plates.
  • Page 34 Select Protocol > Procedure . If prompted to select a reader, select the Synergy H1 and click Select a Plate Type. The assay plate must match the plate type selected in Gen5. Otherwise, the results of the read may be invalid.
  • Page 35 Fill the supply bottle with a sufficient volume of the fluid to be used for the prime and the assay. Insert the appropriate inlet tube into the bottle. In Gen5, select System > Instrument Control > Synergy H1 and click Dispenser tab.
  • Page 36 To save reagent, Gen5 provides the option to purge fluid from the system back into the supply bottle. In Gen5, select and click System > Instrument Control > Synergy H1 Dispenser tab. Select the Dispenser number ( ) associated with the supply bottle.
  • Page 37 Maintenance...
  • Page 38 | Maintenance Preventive Maintenance A general Preventive Maintenance (PM) regimen for all Synergy H1 models includes periodically cleaning all exposed surfaces and inspecting/cleaning the excitation and emission filters and mirrors (if used). For models with the external dispense module, additional tasks include flushing/purging the fluid path, and cleaning the tip prime trough, priming plate, supply bottles, dispense tubing, and injectors.
  • Page 39 Do not immerse the instrument, spray it with liquid, or use a “wet” cloth on it. Do not allow water or other cleaning solution to run into the interior of the instrument. If this happens, contact BioTek’s Technical Assistance Center. Important! Do not apply lubricants to the microplate carrier or carrier track.
  • Page 40 Wipe all exposed surfaces of the dispense module (if used). Wipe all exposed surfaces of the gas controller module (if used). If detergent was used, wipe all surfaces with a cloth moistened with water. Use a clean, dry cloth to dry all wet surfaces. BioTek Instruments, Inc.
  • Page 41 Inspect the glass filters for speckled surfaces or a “halo” effect. This may indicate deterioration due to moisture exposure over a long period of time. If you have any concerns about the quality of the filters, contact your BioTek representative.
  • Page 42 Open the access door on the front of the instrument and slide the filter cube straight out of its compartment. Set the filter cube on the work surface. Using a 7/64" hex wrench, remove the screw and washer located between the emission filter positions ( Figure Figure 10 BioTek Instruments, Inc.
  • Page 43 Figure 11 Flush/Purge the Fluid Path Applies only to Synergy H1 models with injectors. At the end of each day that the dispense module is in use, flush the fluid path using the Gen5 priming utility. Leave the fluid to soak overnight or over a weekend, and then purge the fluid before using the instrument again.
  • Page 44 Empty/Clean the Tip Prime Trough Applies only to Synergy H1 models with injectors. The tip prime trough is a removable cup located in the rear pocket of the microplate carrier, used for performing the tip prime. The trough holds about 1.5 mL of liquid and must be periodically emptied and cleaned by the user.
  • Page 45 Clean the Dispense Tubes and Injectors Applies only to Synergy H1 models with injectors. The Synergy H1’s dispense tubes and injectors require routine cleaning, at least quarterly and possibly more frequently, depending on the type of fluids dispensed. Required Materials •...
  • Page 46 To clean the dispense tubes, soak them in hot, soapy water to soften and dissolve any hardened particles. Flush each tube by holding it vertically under a stream of water. BioTek Instruments, Inc.
  • Page 47 Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the biohazard(s) they handle.
  • Page 48 Do not immerse the instrument, spray it with liquid, or use a “wet” cloth. Do not allow the cleaning solution to run into the interior of the instrument. If this happens, contact the BioTek Service Department. Turn off and unplug the instrument for all decontamination and cleaning operations.
  • Page 49 10.0% NaClO; prepare a 1:20 dilution. Household bleach is typically 5.0% NaClO; prepare a 1:10 dilution. Open the plate carrier door; slide out the plate carrier. Moisten a cloth with the bleach solution or alcohol. Do not soak the cloth. Synergy H1...
  • Page 50 Empty the beaker containing the outlet tubes. Put the tubes back in the empty beaker. If sodium hypochlorite (bleach) was used, perform Rinse the Fluid Lines next. Otherwise (or after performing the Rinse procedure), repeat steps 1–13 for SYRINGE 2/Dispenser 2. BioTek Instruments, Inc.
  • Page 51 If decontaminating in a bleach solution, remove the trough and plate, and thoroughly rinse with DI water. • If decontaminating with alcohol, let the trough and plate air dry. Discard the used gloves and cloths using a biohazard trash bag and an approved biohazard container. Synergy H1...
  • Page 52 | Maintenance BioTek Instruments, Inc.
  • Page 53 Instrument Testing...
  • Page 54 | Instrument Testing System Test Each time the Synergy H1 is turned on, it automatically performs a series of tests on the reader’s motors, lamps, the PMT, and various subsystems. If all tests pass, the reader ejects the microplate carrier and the LED on the power switch remains on.
  • Page 55 If the C6 filter is Holmium glass, the certificate contains two Spectral Bandpass tables. For the Synergy H1, which has a bandpass wider than 5 mm for wavelengths greater than 285 nm and less than 4 nm for 230–285 nm, we...
  • Page 56 Test Plate. The accuracy of the wavelength should be ± 3 nm (± 2 nm instrument, ± 1 nm filter allowance). BioTek Instruments, Inc.
  • Page 57 Do not remove the filter from the Test Plate, and do not use alcohol or other cleaning agents. Make sure the Test Plate is within its calibration certification period. If it is out of date, contact BioTek to schedule a recertification. Check the microplate carrier to ensure it is clear of debris. •...
  • Page 58 Stock Solution formulated by diluting a dye solution available from BioTek (A) or from the ingredients listed below (B). Solution A • BioTek QC Check Solution No. 1 (PN 7120779, 25 mL; or PN 7120782, 125 mL) • Deionized water •...
  • Page 59 Absorbance Liquid Test | Add 0.5 mL of Tween 20, or 5 mL of BioTek’s wetting agent. Fill to 1 liter with DI water; cap and shake well. The solution should measure approximately 2.000 OD when using 200 µL in a flat-bottom microwell.
  • Page 60 | Instrument Testing Fluorescence Liquid Tests For Synergy H1 models with fluorescence capability, BioTek has developed a series of liquid tests for verifying performance. Fluorescence Module Applicable Liquid Test(s) Filter-Based Fluorescence Intensity Corners, Sensitivity, Linearity Monochromator-Based Fluorescence Intensity Corners, Sensitivity, Linearity...
  • Page 61 Fluorescence Polarization test H1_TRF.prt Time-Resolved Fluorescence test For the Monochromator-Based Fluorescence System: H1_M_FI_B_SF.prt Corners, Sensitivity, and Linearity tests, using the Bottom optics H1_M_FI_T_SF.prt Corners, Sensitivity, and Linearity tests, using the Top optics H1_M_FI_T_MUB.prt Alternative top optics test, using methylumbelliferone Synergy H1...
  • Page 62 Phosphate-Buffered Saline (PBS), pH 7.2–7.6 (e.g., Sigma tablets, Mfr. #P4417, or equivalent) and pH meter or pH indicator strips with pH range 4 to 10 • Sodium Fluorescein Powder (1 mg vial, BioTek PN 98155) • If testing both Top and Bottom optics (mono-based fluorescence A new, clean 96-well glass-bottom Greiner SensoPlate (Mfr.
  • Page 63 Corners/Sensitivity/Linearity Tests, in the same microplate. • The recommended test solutions are available from Invitrogen Corporation, in their “FP One-Step Reference Kit” (PN P3088) and from BioTek (PN 7160014). This kit includes: (Green) Polarization Reference Buffer, 15 mL Green Low Polarization Reference, 4 mL Green High Polarization Reference, 4 mL The kit also includes two red polarization solutions;...
  • Page 64 | Instrument Testing Corners/Sensitivity/Linearity Tests: If using BioTek’s sodium fluorescein powder (PN 98155), be sure to hold the vial upright and open it carefully; the material may be concentrated at the top. If a centrifuge is available, spin down the tube before opening.
  • Page 65 Fluorescence Liquid Tests | Fluorescence Polarization (FP) Test The recommended test solutions are available from Invitrogen Corporation and from BioTek. They do not require additional preparation. Time-Resolved Fluorescence (TRF) Test The recommended test solutions are available from Invitrogen Corporation and BioTek.
  • Page 66 • Corners/Sensitivity/Linearity (top optics) and the FP test can be performed using the same solid black plate. • Corners/Sensitivity/Linearity (top optics) for the filter- and monochromator-based fluorescence systems can be performed using the same solid black plate. BioTek Instruments, Inc.
  • Page 67 Do not discard the tips. • Aspirate 150 µL from column 4 and dispense into column 5. Mix the wells using the pipette. • Aspirate 150 µL from column 5. Discard the solution and the tips. Synergy H1...
  • Page 68 SF solutions and buffer. This will dilute the wells as shown below: 0.75 0.375 0.1875 0.0938 0.0469 0.75 0.375 0.1875 0.0938 0.0469 0.75 0.375 0.1875 0.0938 0.0469 0.75 0.375 0.1875 0.0938 0.0469 BioTek Instruments, Inc.
  • Page 69 Pipette 200 µL of deionized water into wells A6–H6. • If you have not already done so, shake the vial of 20 pM europium suspension vigorously for 30 seconds prior to pipetting. • Pipette 200 µL of the 20 pM europium suspension (Eu) into wells A8–B8. Synergy H1...
  • Page 70 Calculate the Detection Limit, in pM: 750 / ((Mean SF – Mean Buffer)/(3 * Standard Deviation Buffer)) To pass, the Detection Limit must be Optic Probe less than or equal to: EX 485 nm, EM 528 nm Top/Bottom: 20 pM (3.76 pg/mL) BioTek Instruments, Inc.
  • Page 71 Calculate the Signal for each LPR well: Subtract the Mean Blank from its measurement value. Using the raw data from the Perpendicular read: • Calculate the Mean Blank (wells A6–H6). • Calculate the Signal for each HPR well: Subtract the Mean Blank from its measurement value. Synergy H1...
  • Page 72 Calculate the Detection Limit, in fM: 20000 / ((Mean Eu – Mean DI water)/(3 * Standard Deviation DI water)) To pass, the Detection Limit Optic Probe must be less than or equal to: Top, with 400-nm 250 fM dichroic mirror BioTek Instruments, Inc.
  • Page 73 Does the Plate Type setting in the Gen5 protocol match the plate you used? • For injector models, spilled fluid inside the reader may be fluorescing, which can corrupt your test results. If you suspect this is a problem, contact BioTek TAC. •...
  • Page 74 If you suspect this is a problem, contact BioTek TAC. • The PMT or optical path(s) may be deteriorating, or the optics or other hardware may be misaligned. Contact BioTek’s Technical Assistance Center. Gen5 Protocol Reading Parameters The information in the following tables represents the recommended reading parameters.
  • Page 75 350 msec Measurements Per Data Point: Lamp Energy: Low (faster) Time-Resolved: Enabled Delay Before Collecting Data: 300 µsec Data Collection Time: 1000 µsec Filter Set: 1 (filter cube) Excitation: 360/40 nm Emission: 620/40 nm Optics Position: Top 400 nm Synergy H1...
  • Page 76 FIRST Read Step All other parameters same as for first read step Read Step: Read Wells: H10–H12 Name: “Corners 4” Gain/Sensitivity: Use first filter set sensitivity from FIRST Read Step All other parameters same as for first read step BioTek Instruments, Inc.
  • Page 77 Luminescence Test Applies only to models with Luminescence capabilities. For Synergy H1 models with luminescence capability, BioTek provides two methods for verifying the performance of the luminescence module. One method measures a Harta Luminometer Reference Microplate, which is an LED-based test plate. Contact BioTek to purchase a plate, or go to www.hartainstruments.com for more information.
  • Page 78 Materials • Harta Luminometer Reference Microplate, PN 8030015 • Harta Plate Adapter for the Synergy H1, PN 8042263 • Gen5 protocol (see page 68) Procedure Turn on the Harta reference plate using the I/O switch on the back of the plate.
  • Page 79 • Glowell, PN GLO-466, formerly available from LUX BioTechnology, Ltd. (www.luxbiotech.com) • Glowell Adapter Plate, available from BioTek, PN 7160006 • Gen5 protocol (see page 68) Procedure Insert the Glowell (“window” side up) into well D8 of the Adapter Plate.
  • Page 80 Background Detection Method: Luminescence Read Type: Endpoint Integration Time: 0:10.00 MM:SS.ss Delay After Plate Movement: 350 msec Dynamic Range: Standard Filter Sets: 1 (filter cube) Excitation: Plug Emission: Hole Optics Position: None Gain/Sensitivity: Read Height: 4.00 mm BioTek Instruments, Inc.
  • Page 81 Costar 96 black opaque Delay Step: 3 minutes Read Step 1: Read Wells: Label: Reference well A2 Detection Method: Luminescence Read Type: Endpoint Integration Time: 0:10.00 MM:SS.ss Delay After Plate Movement: 350 msec Dynamic Range: Standard Gain/Sensitivity: Read Height: 1.00 mm Synergy H1...
  • Page 82 1.00 mm Troubleshooting If either test fails, try the following suggestions. If the test(s) continues to fail, print the results and contact BioTek’s Technical Assistance Center. • Ensure that the reading is performed through a hole in the filter cube, not through a glass filter.
  • Page 83 Is the test failing because the standard deviation of the empty background wells is 0 (resulting in a division-by-zero error when calculating the Signal-to- Noise Ratio)? If yes, try pipetting 100 µL of deionized water into all wells of Columns 9, 10, and 11 (the background wells). Synergy H1...
  • Page 84 Dispense Module Tests Applies only to models with the Dispense module. BioTek Instruments, Inc., has developed a set of tests that you can perform to ensure that the dispense module performs to specification initially and over time. We recommend that you perform these tests before first use (e.g., during the Initial OQ), and then every three...
  • Page 85 1.300 and 1.700 OD at 405/750 nm. The solutions should be at room temperature. If you do not have BioTek’s Green Test Dye Solution (PN 7773003), prepare a dye solution using one of the following methods: Using BioTek’s Blue and Yellow Concentrate Dye Solutions:...
  • Page 86 | Instrument Testing Procedure for Models with Absorbance Capabilities The Synergy H1 Operator’s Manual contains the procedure for models without Absorbance capabilities. If you have not already done so, create Gen5 protocols Disp 1 Test.prt and Disp 2 Test.prt. Prime both dispensers with 4000 µL of deionized or distilled water.
  • Page 87 The injectors may require cleaning (see the Maintenance chapter). • Each dispense module is factory-calibrated for the Synergy H1 it ships with. Verify that the serial number on the dispense module matches the serial number on the reader. Even if the serial numbers match, it is still possible that the calibration values have been inadvertently changed.
  • Page 88 | Instrument Testing Gen5 Test Protocols for Models with Absorbance Capabilities This section contains instructions for creating two Gen5 protocols specifically for performing the Synergy H1 Dispense Precision and Accuracy test. Start by selecting , and add/configure System > Reader Configuration Synergy H1 (if it is not already there).
  • Page 89 Read Speed: Normal Two Wavelengths: 405 and 750 nm Read Step label: “20 and 5 uL Read_Disp 1” (or _Disp 2) Wells: A5..H12 Detection Method: Absorbance Read Type: Endpoint Read Speed: Normal Two Wavelengths: 630 and 750 nm Synergy H1...
  • Page 90 20 and 5 µL Read step at set to the 20 and 5 µL Read step at New Data Set Name resembling ‘Delta OD 20 and 5 ul_Disp <#>’ Formula DS1-DS2 applied to wells BioTek Instruments, Inc.
  • Page 91 Specifications...

  • Page 92: General Specifications

    | Specifications General Specifications Microplates The Synergy H1 accommodates standard 6-, 12-, 24-, 48-, 96-, and 384-well microplates with 128 x 86 mm geometry, and the Take3 and Take3Trio Micro-Volume Plates. Maximum Plate Height: Absorbance mode: plates up to 0.8" (20.30 mm) high Fluorescence, monochromator-based mode: plates up to 0.89"...
  • Page 93 Amplitude: 1 mm to 6 mm in 1-mm steps Frequency : ~10 Hz to ~3 Hz Double Orbital Fast: Amplitude: 1 mm to 6 mm in 1-mm steps Frequency : ~14 Hz to ~5 Hz * Frequency is based on the amplitude selected. Synergy H1...
  • Page 94 Dispensing a 200-µL solution of deionized water, 0.1% Tween 20, and dye at room temperature: < 2.0% for volumes of 50–200 µL < 4.0% for volumes of 25–49 µL < 7.0% for volumes of 10–24 µL < 10.0% for volumes of 5–9 µL BioTek Instruments, Inc.
  • Page 95 96-well and 384-well plate, normal read speed 0–2 OD: ±1% ±0.005 OD, delay after plate movement = 100 ms 2–2.5 OD: ±3% ±0.005 OD, delay after plate movement = 100 ms 96-well and 384-well plate, sweep read speed 0–1 OD: ±2% ±0.010 OD Synergy H1...
  • Page 96 230 to 999 nm λ accuracy ± 2 nm λ repeatability ± 0.2 nm λ bandpass < 4 nm (230–285 nm), < 8 nm (>285 nm) Photodiodes (2). Measurements are reference channel-corrected for Detector light source fluctuation. BioTek Instruments, Inc.
  • Page 97 Fluorescence Specifications (Mono-Based) | Fluorescence Specifications (Mono-Based) The Synergy H1 reads fluorescence intensity with monochromators from the top and bottom of 6- to 384-well plates. All detection limit (DL) requirements are measured by the “two-point” method, which gives the limit of detection at a signal-to-noise ratio of one where noise is defined as three times the standard deviation of the background wells.
  • Page 98 GLOWELL (LUX BioTechnology PN GLO-466 BTI#98243) to the output of a luciferase/ATP/luciferin reaction. Correlation was performed to determine the collection efficiency of the instrument. Production testing is done via the GLOWELL standard. BioTek Instruments, Inc.

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