Luminescence Test - Biotek Synergy H1 Instructions For Use Manual

Read Step: Read Wells:
Name:
Detection Method:
Read Type:
Read Speed:
Delay After Plate Movement:
Measurements Per Data Point:
Lamp Energy:
Wavelengths:
Excitation:
Emission:
Optics Position:
Gain/Sensitivity:
Read Height:

Luminescence Test

Applies only to models with Luminescence capabilities.
For Synergy H1 models with luminescence capability, BioTek provides two methods for
verifying the performance of the luminescence module. One method measures a Harta
Luminometer Reference Microplate, which is an LED-based test plate. Contact BioTek to
purchase a plate, or go to www.hartainstruments.com for more information. The other
method measures a LUX Biotechnology, Ltd., Glowell unit, which is a small, sealed
cylinder with a gaseous tritium light source.
Before using F-LumTest_Harta.prt or F-LumTest_Glowell.prt, create the filter set shown
below.
Synergy H1
Luminescence Test |
C1–F12
"Sensitivity"
Fluorescence
Endpoint
Normal
350 msec
100
Low (faster)
1 (filter cube)
485
528
Top (or Bottom)
Auto, Scale to High Wells, C1, 50000
7.00 mm
65