Results Analysis; Sensitivity Test; Monochromator-Based Fluorescence System - Biotek Synergy H1 Instructions For Use Manual

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| Instrument Testing

Results Analysis

Corners Test
1. Calculate the Mean of the wells containing the 3.3 nM SF test solution (A1–
A3, A10–A12, H1–H3, H10–H12).
2. Calculate the Standard Deviation of the same 12 wells.
3. Calculate the % CV:
The % CV must be

Sensitivity Test

Filter-Based Fluorescence System
1. Calculate the Mean and Standard Deviation for the buffer wells (C10–F12).
2. Calculate the Mean for the 1000 pM (1 nM) SF solution wells (C1–F1).
3. Calculate the Detection Limit, in pM:
1000 / ((Mean SF – Mean Buffer)/(3 * Standard Deviation Buffer))
Top, with 510-nm
dichroic mirror

Monochromator-Based Fluorescence System

1. Calculate the Mean and Standard Deviation for the buffer wells (C10–F12).
2. Calculate the Mean for the 750 pM (0.75 nM) SF solution wells (C1–F1).
3. Calculate the Detection Limit, in pM:
750 / ((Mean SF – Mean Buffer)/(3 * Standard Deviation Buffer))
EX 485 nm, EM 528 nm
(Standard Deviation/Mean) * 100
< 3.0 to pass.
To pass, the Detection Limit
Optic Probe
must be less than or equal to:
Optic Probe
10 pM (2 pg/mL)
To pass, the Detection Limit must be
less than or equal to:
Top/Bottom: 20 pM (3.76 pg/mL)
BioTek Instruments, Inc.