Fluorescence Polarization (Fp) Test; Time-Resolved Fluorescence (Trf) Test; Test Solutions - Biotek Synergy H1 Instructions For Use Manual

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Emission filter 528/20 nm installed
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510-nm dichroic mirror installed

Fluorescence Polarization (FP) Test:

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A new, clean, 96-well solid black microplate, such as Corning Costar #3915. A
Greiner SensoPlate can also be used.
The FP test can be performed in conjunction with the top
Corners/Sensitivity/Linearity Tests, in the same microplate.
•
The recommended test solutions are available from Invitrogen Corporation, in
their "FP One-Step Reference Kit" (PN P3088) and from BioTek (PN 7160014).
This kit includes:
(Green) Polarization Reference Buffer, 15 mL
Green Low Polarization Reference, 4 mL
Green High Polarization Reference, 4 mL
The kit also includes two red polarization solutions; these are not
used.
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Excitation filter 485/20 nm and emission filter 528/20 nm installed
•
510-nm dichroic mirror and polarizers installed

Time-Resolved Fluorescence (TRF) Test:

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15-mL conical-bottom, polypropylene sample tube
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Excitation filter 360/40 nm and emission filter 620/40 nm installed
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400-nm dichroic mirror installed
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A new, clean 96-well solid white microplate, such as Corning Costar #3912.
•
The recommended test solution (FluoSpheres carboxylate-modified
microspheres, 0.2 µm europium luminescent, 2 mL) is available from Invitrogen
Corporation (PN F20881) and from BioTek (PN 7160011).

Test Solutions

Determine which tests you need to run for your reader model, and then prepare
the necessary solutions; see page 49.
Filter the solutions to remove particulates that could cause erroneous
readings. Do not allow dust to settle on the surface of the solution;
use microplate covers or seals when not reading the plate.
Synergy H1
Fluorescence Liquid Tests |
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