Fluorescence Liquid Tests - Biotek Synergy H1 Instructions For Use Manual

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| Instrument Testing

Fluorescence Liquid Tests

For Synergy H1 models with fluorescence capability, BioTek has developed a series of
liquid tests for verifying performance.
Fluorescence Module
Filter-Based Fluorescence Intensity
Monochromator-Based Fluorescence Intensity
Fluorescence Polarization
Time-Resolved Fluorescence
•
The Corners Test
the fluorescence probes.
•
The Sensitivity Test
ability to detect specific compounds at low concentrations ensures that the
monochromator, optical paths, and PMT are all in working order.
•
The Linearity Test
proportionally with changes in concentration. Proving that the system is linear
allows the Sensitivity Test to be run on two points instead of using serial dilutions.
•
The FP Test
solution properly. It verifies the polarizers are installed in the proper orientation,
and the mechanism is in proper order.
•
The TRF Test
optical path, and PMT are all in working order.
The tests presented in this section require specific microplates,
solutions, wavelengths, filters, and mirrors. Your laboratory may
require a deviation from some of these tests. For example, you may
wish to use a different fluorescing solution, dichroic mirror, or
microplate.
If deviation from the tests as presented in this section is required, the
following steps should be taken the first time each test is run:
verifies that the plate carrier is properly aligned in relation to
verifies the fluorescence reading capability of the reader. The
verifies that the system is linear, that is, signal changes
verifies the ability of the instrument to measure polarization of the
verifies the performance of the xenon flash bulb and that the filters,
1 Perform the tests exactly as described on the following pages.
2 Rerun the tests using your particular solutions, filters, mirrors,
microplates, etc. If results are comparable, then the results from these
tests will be your baseline for future tests.
3 Document your new test procedure(s), and save all test results.
Applicable Liquid Test(s)
Corners, Sensitivity, Linearity
Corners, Sensitivity, Linearity
FP
TRF
BioTek Instruments, Inc.