Sutter Instrument P-97 Operation Manual page 22

18
VELOCITY
(Range 0-255)
TIME
(Range 0-255)
DELAY
(Range 0-255)
Special Condition:
Special Condition:
Special Condition:
The VELOCITY of the glass carriage system is measured
as the glass softens and begins to pull apart under a
constant load. The increasing velocity of the initial pull
is determined by the viscosity of the glass, which in turn
is a function of the glass temperature. The adjustable
velocity allows for a selection of a precise glass tempera-
ture as the trip point for the hard pull. Useful values for
velocity range from 10 to 150 with lower values being
used for patch and injection pipettes and higher values
for micropipettes.
VELOCITY = 0
If VEL=0 and PULL=0, the HEAT will be on for the
duration of the TIME programmed (1 unit equals 10ms).
This feature allows you to use the puller in a FIRE
POLISH MODE.
Controls the length of time the cooling air is active.
This parameter is one of two available modes of cooling
If VEL>0 then one unit of TIME represents 1/2ms. If
VEL=0 then one unit of TIME represents 10ms.
TIME = 0
The air solenoid is disabled when TIME=0 (no active
cooling). This allows the pulling of special pipette
shapes. Most often used to pull long tube-like shapes
such as those used for microperfusion.
A cooling mode which controls the delay time
between when the HEAT turns off and when the hard
PULL is activated. The air is automatically turned on
for 300ms. The higher the DELAY value, the cooler the
glass will be when the hard PULL is executed. Thus,
increasing the DELAY results in decreased taper length
and increased tip diameter. If VEL>0 then one unit of
DELAY represents 1/2ms. If VEL=0 then one unit
represents 10ms.
DELAY = 0
The air solenoid is disabled when DELAY = 0 (no active
cooling). This allows the pulling of special pipette
shapes. Most often used to pull long tube-likes shapes
such as those used for microinjection or
microperfusion.