General Information; Technical Support; What ' S New On The P-97 - Sutter Instrument P-97 Operation Manual

GENERAL INFORMATION

The P-97 can fabricate pipettes for use in intracellular recording, patch-clamping, microinjec-
tion and microperfusion. Realizing the full potential of this instrument is dependent on a
complete understanding of the way it implements the pulling process. To this end, we urge
that this manual be read in its entirety. To aid in understanding the function of the instru-
ment, sample programs are already loaded in memory (as discussed in subsequent material).
The Model P-97 Flaming/Brown Micropipette Puller combines a proven pulling technology
with programmability to produce a very versatile instrument. The pulling mechanism is
derived from the P-77/P-80 series of pullers, which have demonstrated the ability to pull a
complete range of pipette profiles. Added to this mechanism is the ability to program differ-
ent pulling sequences; thus, allowing ease of use for pulling a multiplicity of pipettes on one
device.
The P-97 is a 'velocity sensing' puller. This patented feature allows the puller to indirectly
sense the viscosity of the glass, giving the P-97 the ability to pull pipettes from all glasses
except quartz. Even difficult to pull formulations, such as aluminosilicate glasses, are
handled with relative ease.
Throughout this manual reference will be made to the size of the glass tubing used to pull
micropipettes. The convention used here for describing the outside diameter (O.D.) and inside
diameter (I.D.) is as follows: O.D. x I.D. These dimensions will always be given in millimeters
(mm). See our catalog or visit www.sutter.com to find a wide selection of glass capillaries
available for purchase.

Technical Support

Unlimited technical support is provided by Sutter Instrument Company at no charge to our
customers. Our technical support staff is available between the hours of 8:00 AM and 5:00
PM (Pacific Standard Time) at (415) 883-0128. You may also E-mail your queries to
info@sutter.com.
What's new on the P-97?
For users familiar with the P-80PC and/or P-87 pullers, the P-97 provides the advanced
features incorporated into those instruments plus:
· Humidity controlled chamber surrounding the heating filament. Glass is now pulled in a
constant humidity environment to minimize environmental effects on pipette reproduc-
ibility.
· Vacuum fluorescent display for improved readability
· 25% increase in available power
· Programmable air pressure
· Time of pull displayed at the end of each pull sequence
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