Checking The Volume (Calibration) - BRAND Dispensette S Operating Manual

Error limits
Nominal volume ml
1
2
5
10
25
50
100
V
N
.
A
=
T
V
T
i.e.
V
N
V = 50% N
P
V = 10% N
P
*A = Accuracy, CV = Coefficient of Variation
The error limits in DIN EN ISO 8655-5 are significantly lower. The maximum error for a single measure-
ment can be approximated from the sum of error limits EL = A + 2 × CV (for 25 ml: 125 µl+ 2 x µl= 175
µl).

7 Checking the Volume (Calibration)

Depending on use, we recommend that gravimetric testing of the instrument be carried out every 3-12
months. This time frame should be adjusted to correspond with individual requirements. The com-
plete testing procedure (SOP) can be downloaded at www.brand.de. For GLP- and ISO-compliant eval-
uations and documentation, we recommend the EASYCAL™ calibration software from BRAND. Demo
version can be downloaded from www.brand.de. Gravimetric volume testing according to DIN EN ISO
8655- 6 (for measurement conditions, see Error limits, p. 58) is performed as follows:
1. Prepare the instrument
Clean the instrument (Cleaning, p. 62), fill it with distilled H
997490
6 with a completely filled instrument and with uniform and
smooth dispensing.
A* ≤± %
0.5
0.5
0.5
0.5
0.5
0.5
0.5
Partial volume
The percentage values for A and CV are relative to the nomi-
A
N
nal volume (V
(V ).
T
Volume A* ≤± %
25.0 0.5
12.5 1.0
2.5 5.0
μl
5
10
25
50
125
250
500
) and must be converted for partial volumes
N
μl
125
125
125
NOTICE
O and then prime it carefully.
2
7 Checking the Volume (Calibration)
CV* ≤% μl
0.1 1
0.1 2
0.1 5
0.1 10
0.1 25
0.1 50
0.1 100
CV* ≤% μl
0.1 25
0.2 25
1.0 25
Operating manual
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