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nCounter Pro Analysis System User Manual
nCounter Prep Station: Maintenance Dry Run
NOTE:
When conducting maintenance activities, always use appropriate safety
precautions including wearing safety glasses and gloves.
If the Prep Station has been idle for more than a month, it is highly recommended to perform a dry
run on this equipment, preferably several days in advance of the planned run. The purpose of a dry
run is to lubricate hardware which would ordinarily be lubricated by routine use. This will help ensure
that moving parts do not get stuck while processing precious samples and will thus reduce the risk
of losing such samples.
To perform the dry run, you will need the following "blank" reagent components. It is appropriate
to employ used reagents for this protocol.
•
Full set of used pipette tips
•
2x blank prep plates (opened foil on the top, but no green label on the side)
•
2x tip sheaths
•
3x blank 12‐sample strip tubes
•
Blank cartridge
1.
After locating the blank reagents, turn on the Prep Station and wait for it to boot up.
NOTE:
If the instrument has been idle, the "lubricate O‐rings" message will most likely be
present on the Home screen. It is recommended to
to the dry run.
2.
Open the door and load the blank reagents onto the Prep Station.
3.
Close the door, and from the Main Screen choose the "start processing" function.
4.
When you get to the sample designation
screen, process all twelve 'blank' samples. On
the 'Select Protocol' screen, check the box to
launch the Legacy Protocol (Figure 124).
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
82
‐
clean and re
lubricate the O
Figure 124: 'Select Protocol' screen
SEV-00184-01
‐
rings
prior
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