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nCounter Pro Analysis System User Manual
2.
Follow the instructions as directed on the
screen (Figure 121).
3.
To lubricate the O-rings, first use a twisted Kimwipe or lens cloth to "floss" between the nozzles
and remove any grease from the white tip ejector block above the nozzles. If necessary, pull the
tip ejector block down to thoroughly clean the end of it.
Next, take a very small amount of silicone grease (1/3 of a grain of rice) and rub between gloved
thumb and forefinger to yield a sheen of grease. Then, apply greased thumb and forefinger to
the nozzle around the O-ring as shown (Figure 122).
After applying grease to the front and back of
each nozzle, stick a pipette tip on the nozzle
and twist it lightly back and forth, to spread
the grease to the sides of the nozzles.
IMPORTANT:
than the supplied silicone grease on O-
rings.
IMPORTANT:
equipment malfunction. To test before
running samples, perform the
described in the next section.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
80
Do not use anything other
Excessive grease may cause
dry run protocol
Figure 121: The 'Lubricate O-rings—STEP 2'
screen.
Figure 122: Use a finger to apply a small amount
of NanoString silicone grease to the O-rings.
SEV-00184-01
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