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nCounter Pro Analysis System User Manual
When on the 'Select Sample Tubes' screen
(Figure 57), tubes will be shown in three
different colors.
Blue:
Defined. Blue tubes have the required
information associated with them.
Green:
Selected. Green tubes are currently
selected.
IMPORTANT:
selected, all of the info showing on the screen
will overwrite the existing info for all tubes
selected. If you are not using a CDF, it is
recommended that you begin with all tubes
selected to input the common info (such as
FOV), then select each tube separately to apply sample-specific information.
Grey:
Undefined (empty). Grey tubes have no information associated with them, either because they
haven't been selected yet, or they are empty.
A CDF can be created on a personal computer or the Digital Analyzer. Some examples are described
below.
•
Scenario 1: Create a CDF using the CDF Utility on a personal computer. This is the easiest
and recommended method for creating CDFs. See
Personal
Computer.
•
Scenario 2: Create a CDF manually on a personal computer. This is the most practical
scenario if detailed and unique information is required for each sample within a cartridge, but
macros cannot be enabled in Excel because of institution policies. See
on a Personal Computer.
•
Scenario 3: Create a CDF on the Digital Analyzer and enter data for multiple tubes
simultaneously. This approach is very fast but will only allow identification of different
samples by a unique lane number. Sample names will have to be lined up to lane numbers in
the downstream analysis. See
•
Scenario 4: Create a CDF on the Digital Analyzer, enter data for all tubes, and modify
information for one tube. This scenario would be used if most, but not all, data were the same
for samples in a single cartridge. See
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
42
When multiple
tubes
Creating a CDF on the Digital
are
Figure 57: Select Sample Tubes screen
Creating a CDF Using the CDF Utility on a
Creating a CDF on the Digital
Creating a CDF Manually
Analyzer.
Analyzer.
SEV-00184-01
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