nanoString nCounter Pro User Manual page 61

Analysis system
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SEV-00184-01
10.
Place cartridges into the stage position corresponding to the data entered in Steps 2–7 above.
Use the orientation shown in Figure 89, with the barcode closest to the front of the instrument.
Be sure that each cartridge is seated flat in the slot. Close the magnetic clips gently.
Figure 89: Proper orientation of cartridges when placing them into the Digital Analyzer. Close the
magnetic clips gently and be sure to push down on the metal plate above the cartridge after the lid is
closed to ensure the cartridge lays flat.
11.
Shut the instrument door. On the 'Initiate
Imaging' screen, press start.
The 'Counting Cartridge ID' screen will appear
(Figure 90). Once imaging has begun, the door
will remain locked until the system is paused, or
the run is complete.
Once imaging begins, the 'Counting Cartridge
ID' screen will display the following information:
The cartridge ID for the active cartridge (the
cartridge currently being scanned)
Cartridge scan status/progress
o Blue: cartridge scan completed and/or in progress
o Green: cartridge yet to be scanned during the run
o Clear (white): cartridge position for which no data has been defined
Real-time data on the status of data collection
o current time: the current time of day as defined in the system setup utility
o time left (#): the approximate amount of time to complete the active cartridge
o time left (all): the amount of time to complete all cartridges
o finish time: the time of day the run will be finished
Figure 90: The 'Counting Cartridge ID' screen
with information on the current run.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
nCounter Pro Analysis System User Manual
61

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