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nCounter Pro Analysis System User Manual
Centrifuge the prep plates at 2000g for 2 minutes to collect all liquids in the bottom of the wells
prior to loading the prep plates onto the Prep Station deck. After centrifugation, visually inspect
plates to ensure that reagents have collected at the bottom of each well. Press next.
6.
The 'Waste Receptacles' screen will appear
(Figure 18). Remove the liquid waste container
from the combined receptacle and dispose of
the liquid appropriately. See the previous section
on
Waste Removal
Tips should be discarded into the appropriate
waste
stream
organization's laboratory procedures.
If the system is not being used to handle
biohazardous samples, liquid waste may be
disposed of in the sink or other drain. Press next.
7.
The
'Reagent
(Figure 19). Remove the clear plastic lids and
place the prep plates on the deck. Make sure
plates are oriented with the label facing the
operator and that they are flush with the deck
surface by aligning the plate on the positioning
pin and pressing down firmly near the plate
catch (Figure 20). If running 6 or fewer lanes,
only the front plate needs to be loaded onto the
deck. Press next.
NOTE:
If the plate is placed incorrectly, the Prep
Station will pause the protocol until the user
intervenes.
IMPORTANT:
Station will pierce the wells during processing.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
20
for further information.
as
outlined
in
Plate'
screen
will
Do not remove the foil or pierce the wells on the reagent plate. The Prep
the
local
Figure 18: The 'Waste Receptacles' screen.
appear
Figure 19: The 'Reagent Plate' screen.
SEV-00184-01
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