Waters 2998 Operator's Manual page 116

Mobile phase absorbance measured against air or water (Continued)
Absorbance at specified wavelength (nm)
200
Acids and bases
Acetic acid, 2.61
1%
Hydro- 0.11
chloric acid,
0.1%
Phosphoric <0.01 —
acid, 0.1%
Trifluoro- 1.20
acetic acid
Diammo- 1.85
nium
phosphate,
50 mM
Triethy- 2.33
lamine, 1%
Buffers and Salts
Ammonium 1.88
acetate,
10 mM
Ammonium 0.41
bicarbonate,
10 mM
EDTA, 0.11
disodium,
1 mM
HEPES, 2.45
10 mM,
pH 7.6
MES, 2.42
10 mM,
pH 6.0
C-16 Solvent Considerations
205 210 215
2.63 2.61 2.43
0.02 <0.01 —
— —
0.78 0.54 0.34
0.67 0.15 0.02
2.42 2.50 2.45
0.94 0.53 0.29
0.10 0.01 <0.01 —
0.07 0.06 0.04
2.50 2.37 2.08
2.38 1.89 0.90
220 230 240
2.17 0.87 0.14
— — —
— — —
0.22 0.06 <0.02 <0.01 —
<0.01 — —
2.37 1.96 0.50
0.15 0.02 <0.01 —
— —
0.03 0.03 0.02
1.50 0.29 0.03
0.45 0.06 <0.01 —
250 260 280
0.01 <0.01 —
— — —
— — —
—
— — —
0.12 0.04 <0.01
— —
— — —
0.02 0.02 0.02
<0.01 — —
— —