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8/31/2020
front LCD panel displays the current status
1. Contrasting method e.g., TL, FLUO, or SCAN;
and IL SHUTTER status
2. Objective lens and Magnification, see front top
buttons above
3. Illumination and Apertures
4. Camera Ports/Eyepieces, see front top buttons
above
5. Focus control
Note:
Modes of operation
TL
1. press CHG TL
through BF, DIC, and POL
2. adjust intensity (INT) and
aperture (AP) as needed
3. optional, press CHG CS to scan
mode thus shutoff transmitted
light
4. a knurled wheel (arrowhead) on
the left side below the objective
turret adjusts the DIC shear; it's a
rather tight space!
IL/epifluorescence
1. turn on FLUO (fluorescent light source)
2. press the button for the desirable cube, see the front buttons in Controls
3. press Shutter to illuminate specimen
4. adjust the intensity (INT) and field diaphragm (FD) as needed
5. press Shutter to block illumination when done viewing
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
display on the right shows z at 0 µm
with upper focus stop set and lower
focus stop unset
if upper focus stop is unset, display will indicate --.-- mm and
indicates lower focus stop is set
◑
to rotate
Instruction for Leica SP5 confocal microscope
12/16
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