Advertisement
8/31/2020
Using immersion objective lenses
2 ways to switch from DRY to IMM objectives
semi-auto with Mark and Find (recommended)
1. locate the area of interest with a dry objective and focus
2. click
3. click
4. press and hold Z ↓ to lower the turret completely
5. switch immersion objective into light path
6. move the stage to expose the objective so you can apply immersion fluid
7. apply an appropriate amount of immersion fluid
8. select positionN from the drop down list and the stage should move back and bring the
objective to focus
9. click
manual (not recommended)
1. locate the area of interest with a dry objective and focus
2. make sure upper focus stop is set
3. press and hold Z ↓ to lower the turret completely
4. switch immersion objective into light path (see semi-auto procedures above)
5. offset the turret ~15 ° counterclockwise manually and apply a small drop of immersion fluid on
the objective
6. swing the turret back into position
7. press and hold Z ↑ to put the prep back to the previously set focal plane
when finish
1. press and hold Z ↓ to lower the turret completely
2. remove slide
3. at the end of the session, use the provided lens paper to remove the excess immersion fluid from the
front lens
4. switch in the 10× objective
Best practice
always set the upper focus stop whenever you put on a prep, press SET + Z ↑
switch objective lenses via LAS AF software or the right rear buttons on the microscope
if you want to manually switch the objective, DO NOT grab the objective lens barrel, use the turret
instead
Caution: All our immersion objective lenses have aperture or correction collar, grabbing the
barrel can easily move the collar and also strain the lenses.
select the 10× objective at the end of the session
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
to bring up the Mark and Find interface
to record the current position: positionN
via the objective link in LAS AF (recommended)
or at the microscope: press DRY/IMM to switch mode, press either button CHGOBJ ↻
or ↺ to select the desired objective
to dismiss the Mark and Find interface
13/16
Advertisement
