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Summary of Contents for Leica SP8-X
- Page 1 Wellcome Trust Centre for Human Genetics Cellular-Imaging Microscopy Core The Leica SP8-X SMD Confocal Abridged INSTRUCTIONS Page | 1...
- Page 2 Introduction The Leica SP8-X SMD confocal microscope is located in room 00/075, Lab 1. The microscope is suitable for both live cell and fixed specimens on slides, and it will run alongside the old Zeiss LMS 510 MetaHead confocal. The new Leica SP8 has: •...
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Page 3: General Information
Shut down the PicaTech Slave PC last first and switch on the Slave PC last (the Leica SP8 Master PC goes on first). Log in with the same WTCHG account on both PCs. In the Leica LSM software, if you set the WWL to Constant Percent, at say 10% the actual laser power will vary depending on the laser-line selected. - Page 4 The Leica DMI 6000 Microscope Turning the right hand microscope focus control clockwise (away from you) raises the objective (up). Turning the focus control anti-clockwise (towards you) lowers the objective downwards (down). The SmartMove remote control module on the desktop controls the motorised XY stage and the motorised z focus.
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- Page 6 To transfer user hardware control to the Leica DMI 6000 microscope from the LAS software when looking down the microscope, press the diochroic filter selection button (e.g. DAPI) and then ‘shutter’. Also use the fluorescence/transmitted light toggle button on the bottom left side of the microscope.
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- Page 8 SmartMove XYZ Stage Controller Page | 8...
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Page 10: Switch On Procedure
Switch the PC Microscope rocker switch on (triple Green LED switches on the right of the desk) Switch the Scanner Power rocker switch on Note that the Scanner Power must be on at least 30 seconds before you start the Leica SP8 confocal software Switch the Laser Power rocker switch on Turn the Laser Emission key on The White Light Laser (WLL) box will read 70% power by default. -
Page 11: Switch Off Procedure
Note: this controls power to the PicaQuant PC and both PC monitors 12) Switch the epi-fluorescence Xe mercury lamp power box off If you notice or encounter any problems when using the Leica SP8 microscope, please write the details in the SP8 log book provided (A5 black diary). - Page 12 If you have any microscope/imaging problems or queries, just ask a member of the Microscopy Core staff for help. There is additional help and advice on our Core web-pages: http://www.well.ox.ac.uk/microscopy March 2014 . WTCHG Leica SP8-X SMD confocal microscope user guide. This version updated 11 Page | 12...
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