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8/31/2020
Z-stack
Z-Stack panel
focus control is selectable: z-Galvo (default) or z-
Wide
focus (z-Position) can be adjusted with the
microscope controls, Smart Panel and also via
software (see z-drives in the Appendix)
controls
\
z-drives
z-Galvo
z-Wide
*the objective turret i.e., focus is always adjustable with the microscope focus knob and Smart Move, regardless of the z-drive
selection in the software
Acquire a z-stack
1. in Live mode, move deeper into the prep (z is more positive) to where the stack should start, click
Begin to register this z-Position (black: unset, red: set)
2. move towards the outside of the prep to where the stack should stop, click End to register this z-
Position
3. stop scanning, click the z-step size button and then enter the appropriate slice thickness (check setting
slice thickness in
4. select the number of passes to average in the XY panel
5. click Start (lower middle) to collect the stack
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
microscope
Smart Move*
focus knob*
✓
Appendix
for recommendations)
z-drive controls
Smart Panel
✓
✓
✓
min.
range
step size
± 250 µm
42 nm
> 4 mm
49 nm
5/16
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