Leica SP5 II Instructions Manual

8/31/2020
Instructions for the Leica SP5 II laser
scanning confocal microscope
Content: Check-in and Start up
a z-stack • Sequential scan
Check-in and Start up
1. Main Switch Board: switch on PC Microscope, log on and wait until Windows XP is fully loaded
2. Main Switch Board: switch on Scanner Power, wait 5 S
3. launch LAS AF (desktop shortcut),
select the desirable Configuration
and scanner mode, then click ok
Note: Usually, Configuration is
Machine and Activate Resonant
Scanner is unchecked. However,
if the previous user has selected
simulated SP5 or used resonant
scanner, these will become the
default when start up, therefore please check carefully before clicking OK.
4. make sure the condenser is in the "up" position, click Yes to initialize the stage when prompted
this enables Mark and Find, and Tile scan
stage is configured to return to the position before initialization
5. Main Switch Board: when the software finish loading, switch on Laser Power and turn the key clockwise to
On-1 to enable Laser Emission
6. in LAS AF, click on the Configuration Operating step on top left to bring up Hardware Configuration
Laser: check to activate lasers that will be used for the session
Note: If you need the Ar laser, set the power to 20 %. You can use higher power which will
shorten the life span of the laser; but one should never go above 80 % into the red region.
(optional) Settings > Resolution > Bit Depth: select 12 Bit for 4096 grey levels, the maximum this
system can offer
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
• Set up acquistion parameters
• Check out • Appendix
• Optimize acquistion parameters • Acquire
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