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8/31/2020
Appendix
Content
DMI 6000 inverted microscope:
beam splitter spectra
software overview and layout
LAS AF user interface
DMI 6000 inverted microscope
Controls
focus knob on either side of microscope (z-Wide)
commonly used functions are controlled by 5 sets of buttons
1. left rear variable function buttons
CHG TL
and rotate through different modes TL_BF
(bright field), _DIC, _POL (polarization)
CHANGE CS: toggle between confocal SCAN
and the last selected TL mode
COMBI
Z COARSE Z FINE: toggle z control
2. left front: Illumination Manager
TL/IL: toggle between TL and IL (incident-
light i.e., epifluorescence)
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
basic controls
•
working with lif files
◑
: switch to transmitted light, TL
◑
: epifluorescent + DIC
•
modes of operation
•
•
scanner
using immersion objectives
10/16
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