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8/31/2020
ii. select the beam splitter (dichroic)
beam splitter
laser line (nm)
spectra or details for the beam splitters are in the
iii. setup the detector
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
selections for different laser lines
RT 30/70 for
\
backscatter or
reflection
✓
405
✓
458
✓
476
✓
488
✓
514
✓
561
✓
633
select the emission band: drag the lower and upper
bounds or double click the slider to enter the begin
and end wavelengths
Caution: Drag the sliders slowly or else they will
jam!
click on PMT n to adjust PMT Gain* and Offset*;
good starting values are 800 and 0, respectively
select a LUT as default
activate the PMT
Instruction for Leica SP5 confocal microscope
Substrate
488/561/633
✓
Appendix
TD
DD
458/514
✓
✓
✓
✓
✓
✓
RSP
DD
500
488/561
✓
✓
✓
✓
✓
✓
3/16
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