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8/31/2020
more on immersion objective lenses
objective with correction collar (the upper knurled ring with a black dot)
20×/0.7 NA multi immersion objective, currently set
to use oil only
OIL: oil, with or without coverglass
GLYC: glycerol, with or without coverglass
0.17: water, with #1.5 coverglass
W: ca. 3% NaCl, without coverglass
0: water, without coverglass
Note: Please consult facility staff if it is the first time you want to adjust the correction collar, the
poor design of this area of the inverted stand makes it almost impossible to adjust the collar.
objective with iris diaphragm, controlled by turning the aperture collar (knurled ring)
these lens should be used with the iris diaphragm fully open for the highest NA
to fully open the iris turn the collar clockwise slowly until it clicks
the collar should have a little bit of resistance to turning at this fully open position
40×/1.25 NA oil immersion objective
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
63×/1.2 NA water immersion objective, set at 0.17
T+ ←: turn to this direction for higher
temperature or thinner coverglass
0.14 0.16 0.18: water, with specified
coverglass thickness in mm
→ SW: turn to this direction for higher salt
concentration, lower temperature, or thicker
coverglass
63×/1.4 NA oil immersion objective
14/16
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