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8/31/2020
Smart Panel (coming soon)
beam splitter spectra
RT 30/70 is 30% reflection and 70% transmission
Substrate is just plain glass (50/50)
RSP 500 is a 500 nm long pass filter
TD and DD are triple and double dichroics (transmission bands in blue)
TD 488/561/633
DD 488/561
working with lif file
use ImageJ and Bio-Formats plugins, see details on
use the free Leica software
32-bit Windows 7 and XP:
64 bit Windows 7: LAS X from the
scanner
dwell time at several common scan speed settings
line frequency (Hz) horizontal scan duration (s)
10
100
400
600
1000
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
DD 458/514 is similar to the TD 458/514/594 shown
here
using ImageJ
LAS AF Lite
Downloads
dwell time (µs)
max
0.1
84.9
0.01
8.5
0.025
2.1
0.0017
1.4
0.001
0.9
section, or
LAS AF Lite
min
49.7
5.0
1.2
0.8
0.5
15/16
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