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Related Manuals for Leica SP 5 II HCA
Summary of Contents for Leica SP 5 II HCA
- Page 1 Quick Manual How to start and shut down Leica SP 5 II HCA...
- Page 2 1) First things first. Check that the lid of the gas chamber is not attached to the condenser. Even if you plan to use the gas chamber, take the lid off. 2) Turn on the computer. 3) Change the sample holder if you need to change it. Be careful not to drop the small screws used to fasten the sample holder into the motorized stage.
- Page 3 6) Now you can start the software LAS-AF and insert your sample into the sample holder. As the start-up routine moves the stage, it is not recommended to focus into your sample until after the software has fully started. 7) Choose either machine – with pump or machine – without pump according to your needs.
- Page 4 8) Move the turret as down as it goes by z down button on the right side of the microscope and check the stage’s Z displacement. Objective glasses and plates have focus roughly 3,5mm from bottom and Mattek dishes have focus about 1mm above that.
- Page 5 If you have saved your measurement settings, you can load them from Load/Save single setting menu. The user defined settings are saved to your account are last items on the setting list after pre-programmed Leica settings. Alternatively, sequential settings can be loaded from sequential settings.
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Page 6: Shutting Down
2) Put a dish into the adapter. 3) Put a adapter with dish on a stage and put gas chamber on with tubes attached to gas humidifier. Shutting down 1) Save your data. 2) Check from reservation system when microscope has a next reservation. 3) Go to Configuration/Lasers and shut down any lasers not needed by the next user within two hours.