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Related Manuals for Leica Stellaris 8 FALCON
Summary of Contents for Leica Stellaris 8 FALCON
- Page 1 Leica Stellaris 8 FALCON quick manual Version 1.0...
- Page 2 Start-up 1. Check which stage adapter is in place. If you need to change the stage adapter, do it before you start the microscope. The adapter on the left is meant for 96/24 wellplates and 3D-printed sample holders. The adapter on the right is meant for regular objective glasses and 35 mm dishes.
- Page 3 3. Start the computer and login with your credentials 4. Start the Las X software. 5. Chose the appropriate configuration. For the time being this is ‘machine’. 6. Once the software had fully started, you can put in your sample. 7.
- Page 4 If you have DAPI / Hoechst in your sample, turn on the Diode 405 laser. Then turn on the white light laser (WLL). 8. Now get back to the Acquisition side to set up the excitation / detection paradigm. If you have any saved settings, you can load them from the button (A). If you need to make a new scheme you can add new sequences form the (F) button.
- Page 5 Stellaris has two types of detectors, HyD S and HyD X. HyD S Not quite as sensitive for FLIM or photon counting as HyD X, but tolerate more light. Use these for bright channels and when making a coverslip correction. HyD X Use these detectors for dim channels / for photon counting applications / when doing FLIM.
- Page 6 Shutdown and preparing the microscope for the next user 1. Save your data. 2. Check who is coming next to the microscope and what lasers they need 3. Check from IRIS if the next user is coming in two hours or less. If the next user is coming in 2 hours or less, use protocol A, else use protocol B A.
- Page 7 Check from IRIS if the next user needs the microscope heated. If you used heating, leave the heating on, adjusting temperature if needed. If you did not use heating, turn the heating on, adjusting temperature if needed. Shut the computer down. If you used CO turn it off.